一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用

doi:10.12034/j.issn.1009-606X.217230

过程工程学报 ›› 2018, Vol. 18 ›› Issue (1): 171-176.DOI: 10.12034/j.issn.1009-606X.217230

一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用

宋翠^1,2，葛佳^1,2，李秀男²，李强²，陈超^1,2，王鹏³，马光辉²^*

1. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100190；2. 中国科学院大学化工学院，北京 100049； 3. 北京航空材料研究院，北京 100095

收稿日期:2017-05-03 修回日期:2017-06-21 出版日期:2018-02-22 发布日期:2018-01-29
通讯作者: 马光辉 ghma@home.ipe.ac.cn
基金资助:
复杂固-液界面环境中生物大分子在线表征及大纵向力学测量系统;北京市科技计划生命科学领域前沿技术培育

Application of Adjustable Gradient Mobile Phase Control System on Biomacromolecule Characterization

Cui SONG^1,2, Jia GE^1,2, Xiunan LI², Qiang LI², Chao CHEN^1,2, Peng WANG³, Guanghui MA²^*

1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; 2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China; 3. Beijing Institute of Aeronautical Materials, Beijing 100095, China

Received:2017-05-03 Revised:2017-06-21 Online:2018-02-22 Published:2018-01-29
Contact: MA Guang-hui ghma@home.ipe.ac.cn

摘要/Abstract

摘要： 以注射泵为基础，通过硬件与软件结合，研制了一种可进行梯度调节的流动相控制系统，并与石英晶体微天平联接，利用该系统对流动相中的盐浓度和pH值分别进行梯度调节，在石英晶体微天平上实时研究了牛血清蛋白(BSA)吸附洗脱的变化. 结果表明，该流动相控制系统的流量输出误差为1%，流量稳定性为1%，梯度误差为1%，均符合GB/T 26792-2011要求. 随盐浓度增大或pH值降低，BSA逐渐被洗脱，证明该流动相控制系统能精确控制和模拟复杂固液界面.

关键词: 流动相, 梯度, 石英晶体微天平, 生物大分子表征

Abstract: Based on the syringe pumps and cooperated with the developed software, an adjustable gradient mobile phase control system was developed, furthermore the system was connected with quartz crystal microbalance (QCM). Through the gradient adjustion of salt concentration or pH value, the real time adsorption and elution variations of bovine serum albumin (BSA) were investigated. The results showed that the flow error was 1%, the flow stability was 1%, and the gradient error was 1%, which met the standard of GB/T 26792-2011. With the increase of salt concentration or decrease of pH value, the BSA was eluted gradually. These results demonstrated that this system could regulate the gradient and simulate complicated solid?liquid interface environment accurately.

Key words: mobile phase, gradient, QCM, biomacromolecule characterization

宋翠葛佳李秀男李强陈超王鹏马光辉. 一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用[J]. 过程工程学报, 2018, 18(1): 171-176.

Cui SONG Jia GE Xiunan LI Qiang LI Chao CHEN Peng WANG Guanghui MA?. Application of Adjustable Gradient Mobile Phase Control System on Biomacromolecule Characterization[J]. Chin. J. Process Eng., 2018, 18(1): 171-176.

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一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用

Application of Adjustable Gradient Mobile Phase Control System on Biomacromolecule Characterization

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