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›› 2002, Vol. 2 ›› Issue (5): 0-0.

• 5 •    

双碳源交替刺激毕赤酵母高效表达人尿激酶原

马善康, 李强, 顾小勇   

  1. 清华大学化学工程系，北京 100084
  • 出版日期:2002-10-20 发布日期:2002-10-20

Experimental Observation on Cellulosic Biodegradation in Solid State Fermentation

ZHOU Xiao-hong, CHEN Hong-zhang, LI Zuo-huo   

  1. (State Key Lab. Biochem. Eng., Inst. Proc. Eng., Chinese Academy of Sciences, Beijing 100080, China
  • Online:2002-10-20 Published:2002-10-20

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被引次数

17

摘要： 用毕赤酵母表达人尿激酶原，确定了基本的发酵条件：生长阶段pH控制在5.6，表达阶段pH控制在6，生长和表达阶段发酵温度28℃. 研究发现细胞内乙醇氧化酶(AOX)酶活性的变化与目的蛋白尿激酶原(Pro-UK)酶活性的变化存在很好的对应关系，得到一种新的高效表达目的蛋白的调控方法，即交替加入甘油和甲醇，这一双碳源交替刺激法使表达的酶活性比对照提高了70%.

关键词: 毕赤酵母, 发酵, 尿激酶原, AOX酶

Abstract: A mixed medium of steam exploded wheat straw (SEWS) and wheat bran was fermented in solid state by Penicillium decumbens JUA10. Various degraded constituents and activities of enzymes were analyzed. The process of biodegradation of cellulose is actually simultaneous saccharification and fermentation, and feedback inhibition of reducing sugar on cellulose or hemicelluloses may not heavily occur. Biodegradation of cellulose, hemicellulose was not proportional to activities of cellulase and hemicellulase. The compact structure of lignocellulose was the major cause for the low degradation rate of cellulose and hemicellulose. Hemicellulose was degraded even more slowly than cellulose. Starch was degraded easily while lignin was not degraded.

Key words: cellulose, hemicellulose, cellulase, hemicellulase, solid state fermentation (SSF)

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