欢迎访问过程工程学报, 今天是

›› 2003, Vol. 3 ›› Issue (2): 0-0.

• 2 •    

发酵液的组成对纳滤分离谷氨酰胺的影响

李春,刘元帅,李书良,王晓琳,曹竹安   

  1. 清华大学化工系生物化工研究所,北京100084
  • 出版日期:2003-04-20 发布日期:2003-04-20

Effects of Broth Components on Separation of L-glutamine by Nanofiltration

LI Chun, LIU Yuan-shuai, LI Shu-liang, WANG Xiao-lin, CAO Zhu-an   

  1. Inst. Biochem. Eng., Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Online:2003-04-20 Published:2003-04-20

摘要: 研究了谷氨酰胺发酵液中盐离子对纳滤分离提取谷氨酰铵的作用,系统地考察了操作温度和发酵液组成对纳滤分离透过特性的影响,确定了纳滤分离时发酵液最佳稀释倍数和操作温度. 结果表明,二价阳离子通过改变膜面电荷密度对谷氨酸的透过特性产生很大影响,使其截留率降低了8%. 发酵液中较高浓度的(NH4)2SO4使谷氨酸和谷氨酰胺的截留率和透过通量明显降低,但较高浓度的NH4Cl影响较小,这体现了纳滤膜对不同离子排斥力的差异. 当发酵液稀释至谷氨酰胺浓度为1%,pH调至7时,发酵液中的其它盐离子和残糖因浓度较低而对纳滤分离影响不显著.

关键词: 发酵液, 谷氨酰胺, 纳滤分离, 盐离子

Abstract: The effects of salt ions and other components in L-glutamine fermentation broth on the separation of L-glutamine and L-glutamic acid by nanofiltration were investigated and the effects of process operation parameters on permeation properties were studied systematically. The optimum dilution times of fermentation broth and temperature was established. The study also showed that bivalent cations could affect the permeation properties of L-glutamine and L-glutamic acid, and decreased observed rejection of glutamic acid by 8%. Higher concentration of (NH4)2SO4 in broth reduced the observed rejection of L-glutamine and L-glutamic acid and permeation flux greatly, but higher concentration of NH4Cl did not, which demonstrated different repulsion forces of membrane to different ions. When the broth was diluted to 1% for L-glutamine concentration and adjusted to pH=7, other salt ions and residual glucose in broth would not significantly influence L-glutamine separation by nanofiltration due to their low concentrations.

Key words: L-glutamine, broth, nanofiltration separation, salt ions

中图分类号: