Abstract：This work is concerned with the development of a single-step process for purification of lysozyme from chicken egg white solution by expanded-bed adsorption. The bed expansion behavior and liquid-phase axial dispersion in the expanded bed with Streamline SP in XK16 column were examined. The results indicated that the expanded-bed system was highly efficient for protein purification. Static experiments showed that the cation exchanger gave a high binding capacity for lysozyme and the bound protein could be dissociated by increasing liquid-phase ionic strength. Kinetic studies led to the estimation of the effective pore diffusivity of lysozyme and its dependence on liquid phase ionic strength. Then, the expanded bed was applied to the single-step purification of lysozyme from a chicken egg white solution. As a result, a recovery yield of 86.6% and purification factor as high as 62.6 were reached. The study indicates that the expanded bed with Streamline SP is promising for direct lysozyme isolation and purification from crude feedstocks.
2004, Vol. 4 
