转hTNF-α基因聚球藻培养条件的初步研究

›› 2004, Vol. 4 ›› Issue (2): 136-140.

转hTNF-α基因聚球藻培养条件的初步研究

汪晶,康瑞娟,谭天伟,蔡昭铃,丛威

中国科学院过程工程研究所生化工程国家重点实验室

出版日期:2004-04-20 发布日期:2004-04-20

Preliminary Investigation on the Culture Conditions of Transgenic Synechococcus sp. PCC7002 with hTNF-α Gene

WANG Jing,KANG Rui-juan,TAN Tian-wei,CAI Zhao-ling,CONG Wei

中国科学院过程工程研究所生化工程国家重点实验室

Online:2004-04-20 Published:2004-04-20

摘要/Abstract

摘要： 以转hTNF-α基因聚球藻7002为对象，在摇瓶培养下对其生长条件进行了初步研究. 结果表明，当光照强度为100 μmol/(m2×s)时，藻细胞生长速率最大；35oC为其最佳培养温度；氯化钠浓度为12~24 g/L生长良好，最适浓度为24 g/L；转hTNF-α基因的聚球藻7002能利用铵盐和硝酸盐为氮源，其中硝酸盐对其生长最有利，硝酸钠最适浓度为1 g/L；加入有机碳源可以显著促进藻细胞生长，其中5 g/L的蔗糖对其促进作用最明显.

关键词: 转基因聚球藻7002, 培养条件, 人肿瘤坏死因子-α

Abstract: Light, temperature, NaCl concentration, nitrogen sources, organic carbon source are important factors affecting the growth of transgenic Synechococcus sp. PCC7002 with hTNF-α gene. The growth of transgenic Synechococcus sp. PCC7002 was saturated at the level of 100 μmol/(m2×s) light intensity. The optimal temperature for cultivation was 35oC. The range of NaCl concentration suitable to the cell growth was from 12 g/L to 24 g/L, and the optimal was 24 g/L. Transgenic Synechococcus sp. PCC7002 could use ammonium or nitrate for its nutritional requirement, but nitrate was the preferred nitrogen source and the optimal concentration was 1 g/L NaNO3. Several organic carbon compounds such as sucrose, glucose, glucosamine were tested. It was shown that organic carbon source promoted the cell growth apparently and 5 g/L sucrose was proved to be the most suitable.

Key words: transgenic Synechococcus sp. PCC7002, cultivation condition, hTNF-α

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汪晶;康瑞娟;谭天伟;蔡昭铃;丛威. 转hTNF-α基因聚球藻培养条件的初步研究[J]. , 2004, 4(2): 136-140.

WANG Jing;KANG Rui-juan;TAN Tian-wei;CAI Zhao-ling;CONG Wei. Preliminary Investigation on the Culture Conditions of Transgenic Synechococcus sp. PCC7002 with hTNF-α Gene[J]. , 2004, 4(2): 136-140.