关键词: 白蛋白纳米球, 制备, 体外释药, 丝裂霉素

Abstract: Mitomycin-loaded albumin nanospheres sized between 60 and 100 nm were prepared by the ultrasonic emulsification-crosslinking method. After the albumin aqueous phase containing mitomycin was emulsified into the oil phase by the ultrasonifier to obtain the nanodroplets, glutaraldehyde was added to crosslink the nanodroplets. The optimal emulsifier was chosen by comparing the stability of emulsion prepared with various emulsifiers. It was found that the most stable W/O emulsion was obtained when Arlacel 83 was used as the emulsifier. The influence of the amount of glutataldehyde on the properties of the albumin nanospheres was investigated. It was found that the yield and drug content of nanospheres and loading efficiency of the drug increased, while the diameter and swelling degree of nanospheres, and the rate of drug release in vitro decreased, with the increase of the amount of gluataraldehyde. The optimal amount of glutaraldehyde corresponds to the ratio of aldehyde group to amino group being 16:1. The drug release in vitro can be divided into two phases, both of which can be expressed with the Higuchi equation.

Key words: albumin nanospheres, preparation, in vitro drug release, mitomycin