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›› 2005, Vol. 5 ›› Issue (2): 183-187.

• 生化工程专栏 • 上一篇    下一篇

壳聚糖载体柔性固定化木瓜蛋白酶

魏荣卿,沈斌,刘晓宁,汪海萍,王宇星,欧阳平凯   

  1. 南京工业大学制药与生命科学学院
  • 出版日期:2005-04-20 发布日期:2005-04-20

Flexible Immobilized Papain on the Chitosan Support

WEI Rong-qing,SHEN Bin,LIU Xiao-ning,WANG Hai-ping,WANG Yu-xing,OUYANG Ping-kai   

  1. College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology
  • Online:2005-04-20 Published:2005-04-20

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摘要： 用酶柔性固定化模型，以壳聚糖为载体，双醛淀粉为柔性链，对木瓜蛋白酶进行柔性固定化. 通过对固定化条件的优化，得出选用壳聚糖、双醛淀粉制得的柔性载体(Chitosan-DAS50)在酶用量为14.4 mg/g(酶/干球)、pH 8的条件下，固定木瓜蛋白酶18 h，所得的固定化酶活力回收率达72%，相当于采用壳聚糖-戊二醛(Chitosan-GA)手臂载体的3倍. 结果表明，酶的柔性固定化模型可以改善传统共价结合法固定化及手臂固定化酶活力回收率不高的缺陷.

关键词: 柔性固定化, 壳聚糖, 双醛淀粉, 固定化木瓜蛋白酶

Abstract: In this work "Flexible Immobilized Enzyme" model is adopted. Chitosan-DAS (Dialdehyde Starch) flexible support is synthesized by coupling flexible chain (DAS) onto the surface of chitosan support, then papain is immobilized on this flexible support. Through optimizing immobilization conditions, it is found that when Chitosan-DAS50 is taken as support with the conditions of 14.4 mg/g (enzyme/dry support) and pH 8, immobilization time is 18 h, the recovery of immobilized enzyme activity can reach 72%, being equal to triple of Chitosan-GA (glutaric aldehyde) support. This conclusion has showed that "Flexible Immobilized Enzyme" model can improve recovery of immobilized enzyme activity over traditional covalent immobilization and arm spacer immobilization.

Key words: flexible immobilization, chitosan, dialdehyde starch, immobilized papain