关键词: 混合反胶团, 非离子表面活性剂, 含水量, 无机盐, 溶菌酶溶解性

Abstract: A new mixed reversed micellar system composed of the anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate(AOT) and nonionic surfactant poly(oxyethylene) nonylether(OPE4 with polymerization degree of 4) was prepared and the solubilization of lysozyme in the mixed reversed micelles was investigated under different conditions. The results exhibited that this reversed micelle system had more water content, and the water content was kept to be constant at a wider concentration ratio of AOT to OPE4, which could be ascribed to the change of micellar conformation and electrostatic interaction. The ion species and ionic strength had significant influence on water content in the organic phase, and further changed the solubility of lysozyme in the organic phase. These were caused by the influence of salt ions on double diffuse-layer and repulsion of hydration membrane. Both reducing pH value and increasing surfactant concentration were all beneficial to solubility of lysozyme in the organic phase.

Key words: mixed reversed micelle, nonionic surfactant, water content, salt, lysozyme solubility