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›› 2006, Vol. 6 ›› Issue (3): 462-465.

• 生化工程专栏 • 上一篇    下一篇

Biodegradation of Leather Waste by Enzymatic Treatment

Muhammad Nauman Aftab,Abdul Hameed,Ikram-ul-Haq   

  1. 巴基斯坦伊斯兰堡Quaid-I-Azam大学
  • 出版日期:2006-06-20 发布日期:2006-06-20

Biodegradation of Leather Waste by Enzymatic Treatment

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  1. Dept. Biol. Sci., Quaid-i-Azam Univ., Islamabad, Pakistan
  • Online:2006-06-20 Published:2006-06-20

摘要: The treatment of shavings, trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes alkaline protease produced by Bacillus subtilis in our laboratory by submerged fermentation. Optimum conditions of pH, time duration, temperature and concentration of enzyme were determined for maximum degradation of leather waste. The amount of degradation was measured by the release of amino acid hydroxyproline. Amino acid composition in the hydrolysate obtained by the enzyme hydrolysis was determined. This relative simple biotreatment of leather waste may provide a practical and economical solution.

关键词: leather waste, biodegadation, Bacillus subtilis, alkaline protease

Abstract: The treatment of shavings, trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes alkaline protease produced by Bacillus subtilis in our laboratory by submerged fermentation. Optimum conditions of pH, time duration, temperature and concentration of enzyme were determined for maximum degradation of leather waste. The amount of degradation was measured by the release of amino acid hydroxyproline. Amino acid composition in the hydrolysate obtained by the enzyme hydrolysis was determined. This relative simple biotreatment of leather waste may provide a practical and economical solution.

Key words: leather waste, biodegadation, Bacillus subtilis, alkaline protease

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