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›› 2007, Vol. 7 ›› Issue (1): 145-148.

• 生化工程专栏 • 上一篇    下一篇

利用13C标记葡萄糖分析g-聚谷氨酸的代谢途径

石宁宁,徐虹,姚俊,王军   

  1. 南京工业大学制药与生命科学学院
  • 出版日期:2007-02-20 发布日期:2007-02-20

Investigation of Metabolic Routes to Poly(glutamic acid) by 13C-labeled Glucose as Medium Carbon Source

SHI Ning-ning,XU Hong,YAO Jun,WANG Jun   

  1. College of Life Science and Pharmacy , Nanjing University of Technology
  • Online:2007-02-20 Published:2007-02-20

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摘要： 本实验室筛选的菌株Bacillus subtilis NX-2以葡萄糖和谷氨酸共同作为碳源生产g-聚谷氨酸(g-PGA). 为探讨这2种碳源在g-PGA合成中的作用，在培养基中加入标记的[U-13C]-葡萄糖，检测产物g-聚谷氨酸的核磁共振碳谱信号强度，从而计算葡萄糖代谢进入产物的量. 在培养基中葡萄糖浓度为4%时，g-PGA的碳骨架中由葡萄糖进入的比例为9%左右. 当葡萄糖浓度为3%时，由葡萄糖进入g-PGA的比例降至6%. 证明葡萄糖主要用于能量代谢和菌体合成，只有少量参与g-PGA合成，而谷氨酸为g-PGA单体的主要来源.

关键词: γ-聚谷氨酸, 代谢途径, 核磁共振

Abstract: Bacillus subtilis NX-2 produce was used to g-poly(glutamic acid) (g-PGA) with glucose and glutamic acid as medium carbon sources. To clarify the role of these carbon sources in g-PGA synthesis, metabolic routes to g-poly glutamic acid were investigated using 13C-labeled glucose as medium carbon source by detecting 13C-NMR peak intensity. When the medium glucose concentration was 4%, the repeat units of g-PGA formed from glucose were approximately 9%. While the medium glucose concentration was 3%, the repeat units formed from glucose fell to 6%. Glucose was mainly used to form biomass and provide adenosine tri-phosphate (ATP) for g-PGA synthesis, while glutamic acid was the main source of g-PGA repeat units.

Key words: γ-poly(glutamic acid), metabolic routes, NMR