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›› 2007, Vol. 7 ›› Issue (2): 375-379.

• 生化工程专栏 • 上一篇    下一篇

霍山石斛类原球茎在气升式反应器中补料培养合成多糖

魏明,姜绍通,罗建平   

  1. 合肥工业大学生物与食品工程学院
  • 出版日期:2007-04-20 发布日期:2007-04-20

Fed-batch Cultivation of Protocorm-like Bodies of Dendrobium huoshanense for Producing Polysaccharides in an Air-lift Bioreactor

WEI Ming,JIANG Shao-tong,LUO jian-ping   

  1. Studies on the Characteristic and Kinetics for Suspension Cultures of Protocorm-Like Bodies from Dendrobium huoshanense
  • Online:2007-04-20 Published:2007-04-20

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被引次数

15

摘要： 在10 L气升式反应器中进行了霍山石斛类原球茎悬浮培养. 研究了接种量、通气量、碳、磷等营养成分对霍山石斛类原球茎生长和多糖合成的影响. 在接种量为100 g/L(鲜重)、通气量为0.5 L/min时，30 g/L的蔗糖、2.5 mmol/L的磷酸盐有利于类原球茎生长，而0.312 mmol/L的磷酸盐有利于多糖积累. 根据霍山石斛类原球茎生长和多糖积累的动力学特性，提出了两步培养方式，采用补料策略，确定了最佳补料时间. 结果表明，采用两步培养，生物量达46.7 g/L(干重)，是一步培养的1.5倍，多糖产率达8.15 g/L，是一步培养的2.6倍.

关键词: 霍山石斛, 类原球茎, 气升式反应器, 多糖, 补料培养

Abstract: The cultivation of protocorm-like bodies of Dendrbium huoshanense was carried out in a 10 L air-lift bioreactor. The effects of inoculum size, aeration rate, carbon, phosphate sources on the cell growth and accumulation of polysaccharides were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L (fresh weight) cells and aeration rate of 0.5 L/min. The optimal concentrations of sucrose and phosphate in the medium for the cell growth were 30 g/L and 2.5 mmol/L, respectively. A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides. The results in two-step cultivation indicated that the maximum biomass and polysaccharides yield were 46.7 g/L (dry weight) and 8.15 g/L, which are 1.5 and 2.6 fold of those in one-step cultivation respectively.

Key words: Dendrobium huoshanense, protocorm-like bodies, air-lift bioreactor, polysaccharides, fed-batch cultivation