以恶臭假单胞菌发酵制备D-氨基葡萄糖酸

›› 2009, Vol. 9 ›› Issue (1): 123-127.

以恶臭假单胞菌发酵制备D-氨基葡萄糖酸

袁静孟笑何冰芳

南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院江苏省生物工业技术重点实验室

收稿日期:2008-06-18 修回日期:2008-08-21 出版日期:2009-02-20 发布日期:2009-02-20
通讯作者: 何冰芳

Production of D-Glucosaminic Acid by Fermentation with Pseudomonas putida

YUAN Jing MENG Xiao HE Bing-fang

College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology, The Provincial Key Laboratory of Industrial Biotechnology

Received:2008-06-18 Revised:2008-08-21 Online:2009-02-20 Published:2009-02-20
Contact: HE Bing-fang

摘要/Abstract

摘要： 从土样中分离到D-氨基葡萄糖酸高产菌株GNA5，综合Biolog细菌自动鉴定系统与16S rDNA序列分析结果，鉴定为恶臭假单胞菌(Pseudomonas putida). P. putida GNA5能耐受较高浓度底物D-氨基葡萄糖，可发酵积累D-氨基葡萄糖酸. 初步优化培养基为(g/L)：D-氨基葡萄糖盐酸盐30，葡萄糖5，尿素5, KH2PO4 2, MgSO4×7H2O 0.5, CaCO3 10, pH 7.0. 菌株GNA5在该培养基中发酵48 h可制备25.5 g/L的D-氨基葡萄糖酸，摩尔转化率达93.91%. 自行筛选的P. putida GNA5显示了底物转化能力强及产物分解能力弱的特点，具有进一步产业化开发的潜力.

关键词: D-氨基葡萄糖酸, 氧化发酵, 恶臭假单胞菌, D-氨基葡萄糖

Abstract: Strain GNA5 producing D-glucosaminic acid from D-glucosamine was isolated from soil samples. It was identified as Pseudomonas putida based on the assay of Biology Automated Microbiology Identification System and analysis of 16S ribosomal DNA sequence. P. putida GNA5 could grow in the medium containing high concentration of D-glucosamine, and D-glucosaminic acid was stably accumulated in the oxidative fermentation process. The preliminary optimized culture medium composition (g/L) for the production of D-glucosaminic acid with P. putida GNA5 was obtained as follows: D-glucosamine×HCl 30, glucose 5, urea 5, KH2PO4 2, MgSO4×7H2O 0.5, CaCO3 10, pH 7.0. After 48 h fermentation in this medium, the concentration of D-glucosaminic acid reached 25.5 g/L, with molar yield of 93.9%. P. putida GNA5 exhibited efficient conversion ability of D-glucosamine to D-glucosaminic acid and low degradation ability of D-glucosaminic acid. It shows considerable economic significance in development of D-glucosaminic acid producing process.

Key words: D-glucosaminic acid, oxidative fermentation, Pseudomonas putida, D-glucosamine

中图分类号:

TQ92

袁静孟笑何冰芳. 以恶臭假单胞菌发酵制备D-氨基葡萄糖酸[J]. , 2009, 9(1): 123-127.

YUAN Jing MENG Xiao HE Bing-fang. Production of D-Glucosaminic Acid by Fermentation with Pseudomonas putida[J]. , 2009, 9(1): 123-127.

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