欢迎访问过程工程学报, 今天是

›› 2009, Vol. 9 ›› Issue (1): 190-199.

• 综述 • 上一篇    下一篇

生物转化法生产L-苯基乙酰基甲醇的研究进展

李丹 林建群 林慧彬 林建强 曲音波   

  1. 山东大学微生物技术国家重点实验室 山东大学微生物技术国家重点实验室 山东省中医药研究院 山东大学，生命科学学院，微生物技术国家重点实验室 山东大学，生命科学学院，微生物技术国家重点实验室
  • 收稿日期:2008-09-17 修回日期:2008-11-17 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 林建强

Research Progress in L-Phenylacetylcarbinol Production by Biosynthesis

LI Dan LIN Jian-qun LIN Hui-bin LIN jian-qiang QU Yin-bo   

  1. State Key Laboratory of Microbial Technology, Shandong Universit State Key Laboratory of Microbial Technology, Shandong Universit Shandong Institute of Traditional Chinese Medicine, State Key Laboratory of Microbial Technology, Shandong Universit State Key Laboratory of Microbial Technology, Shandong Universit
  • Received:2008-09-17 Revised:2008-11-17 Online:2009-02-20 Published:2009-02-20
  • Contact: LIN jian-qiang

PDF

摘要： 综述了L-苯基乙酰基甲醇(L-PAC)生物转化机理、菌种选育、转化体系选择、生产过程优化、下游处理等方面的研究进展. 目前，主要采用具有高活性的丙酮酸脱羧酶的Saccharomyces属和Candida属菌株，以苯甲醛和丙酮酸为底物转化生产L-PAC. 生成副产物和底物苯甲醛的毒性是限制产量提高的主要因素. 通过菌株筛选和选育可减少副产物生成并提高菌株耐受性. 通过控制及优化转化过程参数、分批补加底物、采取固定化细胞或固定化酶措施及建立适宜的两相转化体系，能有效提高产量.

关键词: L-苯基乙酰基甲醇, 生物转化, 麻黄碱

Abstract: The research progress in biotransformation of L-phenylacetylcarbinol (L-PAC), including mechanism of biocatalysis, strain screening, biotransformation system, process parameters and downstream processing, was reviewed. Strains belonging to the genera of Saccharomyces and Candida I with high enzyme activity of pyruvate decarboxylase are widely used in biotransformation of L-PAC from benzaldehyde and pyruvate. The formation of by-products and toxicity of substrate significantly influence the production of L-PAC. By strain screening and breeding, suitable strains with high tolerance to benzaldehyde and effective reduction of byproducts can be obtained. By applying the process optimization methodologies of fed-batch culture, process control and process parameter optimization, utilization of cell or enzyme immobilization and adoption of aqueous and organic two-phase system, higher L-PAC production can be achieved.

Key words: L-phenylacetylcarbinol, biotransformation, ephedrine

中图分类号: