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›› 2009, Vol. 9 ›› Issue (4): 786-790.

• 生化工程专栏 • 上一篇    下一篇

半胱氨酸添加与溶氧控制及pH胁迫相结合促进产朊假丝酵母合成谷胱甘肽

梁国斌 堵国成 陈坚   

  1. 江苏技术师范学院化学化工学院,江苏,常州, 213001 江南大学工业生物技术教育部重点实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2009-03-09 修回日期:2009-05-07 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 梁国斌

Enhancement of Glutathione Production by Combining Cysteine Addition with Dissolved Oxygen Control and Low pH Stress in High Cell Density Cultivation of Candida utilis

LIANG Guo-bin, DU GUo-cheng, CHEN Jian,   

  1. School of Chemistry and Chemical Engineering, Jiangsu Teachers University of Technology Lab of Environmental Biotechnology, School of biotechnology Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2009-03-09 Revised:2009-05-07 Online:2009-08-20 Published:2009-08-20
  • Contact: LIANG Guo-bin,

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被引次数

12

摘要： 采用产朊假丝酵母，在细胞高密度培养基础上，考察了半胱氨酸添加与溶氧控制及低pH胁迫对谷胱甘肽(GSH)合成的影响. 结果表明，细胞高密度培养结束时(45 h)，一次性添加50 mmol/L半胱氨酸，第60 h时GSH产量达1534 mg/L. 添加半胱氨酸后的3 h内溶氧控制在5%，之后的12 h将溶氧控制在20%，可减少半胱氨酸添加量，而GSH产量却提高13%. 将30 mmol/L半胱氨酸分2次添加并与pH胁迫和溶氧控制相组合，发酵结束时(78 h)，GSH终产量达1936 mg/L.

关键词: 谷胱甘肽, 产朊假丝酵母, 溶氧控制, pH胁迫

Abstract: The effects of cysteine addition coupled with dissolved oxygen (DO) control and pH stress on glutathione (GSH) production in high cell density (HCD) cultivation of Candida utilis were investigated. By applying HCD cultivation, cell concentration reached 102 g/L and GSH yield was 981 mg/L after 45 h fermentation. At 45 h, single-shot addition of cysteine was utilized to increase GSH production, the results indicated that the 50 mmol/L cysteine addition achieved a maximal GSH yield of 1534 mg/L after 60 h cultivation. In addition, the method of two-step DO control after cysteine addition, by which DO was controlled at 5% in the first 3 h and at 20% in the following 12 h, was developed. As a result, cysteine addition was decreased from 50 to 30 mmol/L, while GSH yield reached 1734 mg/L at 60 h. Moreover, by adopting the strategy of combining two-shot cysteine addition (15 mmol/L for each time) with two-step DO control and low pH stress, a maximal GSH yield of 1936 mg/L was achieved at 60 h.

Key words: glutathione, Candida utilis, dissolved oxygen control, pH stress

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