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›› 2012, Vol. 12 ›› Issue (2): 288-292.

• 生化工程专栏 • 上一篇    下一篇

地衣芽孢杆菌P-104发酵生产g-聚谷氨酸条件优化

张业伟 魏雪团 胡中波 罗明芳 徐林 刘会洲   

  1. 中国科学院研究生院 中国科学院过程工程研究所分离科学与工程实验室 中国科学院研究生院 中国科学院微生物研究所 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中国科学院过程工程研究所分离工程与工程青年实验室
  • 收稿日期:2012-01-09 修回日期:2012-03-01 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 张业伟

Optimization of g-Polyglutamic Acid Production by Bacillus licheniformis P-104

ZHANG Ye-wei WEI Xue-tuan HU Zhong-bo LUO Ming-fang XU Lin LIU Hui-zhou   

  1. Graduate University of Chinese Academy of Sciences Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Institute of Process Engineering, Chinese Academy of Sciences
  • Received:2012-01-09 Revised:2012-03-01 Online:2012-04-20 Published:2012-04-20
  • Contact: ZHANG Ye-wei

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摘要： 对地衣芽孢杆菌P-104发酵合成g-PGA的条件(接种时间、接种量和培养基组成等)进行了优化，并在发酵罐中进行了批式发酵实验. 结果表明，该菌可利用合成培养基生产较高浓度超高分子量(大于2500 kDa)的g-PGA，最佳培养基组分为(g/L)：葡萄糖 80，谷氨酸钠 70，柠檬酸钠 10, (NH4)2SO4 10, MnSO4 0.15, MgSO4 0.8, K2HPO4 0.6, NaNO3 4. 接种时间与量分别为8 h和3%(j)、初始pH 7.5条件下，37℃下180 r/min摇瓶培养24 h，发酵液中g-PGA浓度可达44.7 g/L，比生产速率为1.49 g/(L×h)，是已报道的同类比生产速率的2倍. 采用优化培养基在6.6 L发酵罐中批式发酵培养33 h, g-PGA浓度为32 g/L，比生产速率为0.97 g/(L×h).

关键词: 地衣芽孢杆菌, g-聚谷氨酸, 优化, 批式发酵

Abstract: The fermentation conditions for g-polyglutamic acid (g-PGA) production with Bacillus licheniformis P-104 were optimized in flasks, and the mesoscale batch fermentation was performed in a 6.6 L fermentor. The results showed that high yield of g-PGA with ultra high molecular weight (over 2500 kDa) was obtained under the optimal conditions (g/L): glucose 80, sodium glutamate 70, sodium citrate 10, (NH4)2SO4 10, MnSO4 0.15, MgSO4 0.8, K2HPO4 0.6, NaNO3 4, inoculation time 8 h, inoculum amount 3%(j), initial pH value 7.5, 37℃, and 180 r/min. In the flask fermentation, the maximum g-PGA yield reached 44.7 g/L for 24 h, and the highest specific productivity was 1.49 g/(L×h), being twice of the ultra high molecular weight g-PGA reported previously. In a 6.6 L fermentor, 32 g/L g-PGA was obtained for 33 h with a specific productivity of 0.97 g/(L×h).

Key words: Bacillus licheniformis, g-polyglutamic acid, optimization, batch fermentation

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