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›› 2012, Vol. 12 ›› Issue (6): 1008-1013.

• 生化工程专栏 • 上一篇    下一篇

一株产2-酮基-D-葡萄糖酸菌的筛选鉴定及其发酵优化

牛盼清 杨爱华 杨松鑫 刘立明 陈坚   

  1. 江南大学食品科学与技术国家重点实验室 江南大学食品科学与技术国家重点实验室 江苏省无锡市江南大学生物工程学院 江南大学生物工程学院环境生物技术实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2012-08-20 修回日期:2012-11-08 出版日期:2012-12-20 发布日期:2012-12-20
  • 通讯作者: 刘立明

Screening and Identification of 2-Keto-D-gluconic Acid-producing Strain

NIU Pan-qing YANG Ai-hua YANG Song-xin LI Zhen CHEN Jian,   

  1. State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2012-08-20 Revised:2012-11-08 Online:2012-12-20 Published:2012-12-20
  • Contact: LI Zhen

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被引次数

7

摘要： 以(NH4)2SO4为底物氮源，采用平板变色圈法从土壤中分离筛选出一株2-酮基-D-葡萄糖酸生产菌株Serratia sp. FMME043，对其碳源、氮源、无机源和摇瓶类型等产酸条件进行优化，确定以(NH4)2SO4为氮源的摇瓶产酸最佳条件为葡萄糖180 g/L, (NH4)2SO4 2.0 g/L, KH2PO4 1 g/L，在初始pH 7.0及750 mL双刺摇瓶装液量10%、培养温度30℃、摇床转速200 r/min条件下发酵48 h，2-酮基-D-葡萄糖酸产量达169.5 g/L，得率为0.87 mol/mol，并在7 L发酵罐中进行了验证.

关键词: 2-酮基-D-葡萄糖酸, (NH4)2SO4, 沙雷氏菌属, 发酵优化

Abstract: A 2-keto-D-gluconic acid (2-KGA) producing strain Serratia sp. FMME043 was isolated from the soil by the method of flat discolor circle with (NH4)2SO4 as nitrogen source. The optimal conditions, glucose 180 g/L, (NH4)2SO4 2.0 g/L and KH2PO4 1 g/L, were obtained through one-factor experimental design. Furthermore, the highest 2-KGA concentration 169.5 g/L and yield 0.87 mol/mol were achieved under the optimum conditions with initial pH 7.0, liquid volume 75 mL in a 750 mL flask, culture temperature 30℃, and shaker rotating speed 200 r/min. These results were also confirmed in a 7 L fermentor.

Key words: 2-Keto-D-gluconic acid, (NH4)2SO4, Serratia marcescens, fermentation optimization

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