关键词: 大肠杆菌, 蛋白表达速度, 调控策略, 外源蛋白

Abstract: Based on each stage of the protein expression process, current regulation strategies of the expression rate of transcription, translation and folding of heterologous proteins are introduced and evaluated respectively. Based on the fact that these strategies have different action stages, features, advantages and disadvantages, the idea of combining such strategies to respectively regulate each step of protein expression including transcription, translation and folding is proposed in order to obtain an appropriate overall expression rate and optimize the effect of expression, so as to improve the production efficiency and product quality of heterologous proteins in E. coli system and further its applicability and economy.

Key words: Escherichia coli, protein expression rate, regulation strategy, heterologous protein