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›› 2014, Vol. 14 ›› Issue (3): 482-486.

• 生化工程专栏 • 上一篇    下一篇

丙酸高产菌株的选育及发酵培养基优化

陈唤蛟 李小连 李彦良 王自强 王云山 张利平 苏志国   

  1. 河北大学生命科学学院 河北大学生命科学学院 河北大学生命科学学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 河北大学生命科学学院 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2014-03-13 修回日期:2014-05-05 出版日期:2014-06-20 发布日期:2014-06-20
  • 通讯作者: 陈唤蛟

Screening of High Yield Propionic Acid Producing Strain and Optimization of Its Fermentation Medium

CHEN Huan-jiao LI Xiao-lian LI Yan-liang WANG Zi-qiang WANG Yun-shan， ZHANG Li-ping SU Zhi-guo   

  1. College of Life Science, Hebei University College of Life Science, Hebei University College of Life Science, Hebei University Key Lab of Biochemical Engineering, Institute of Process Engineering, State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences College of Life Science, Hebei University State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
  • Received:2014-03-13 Revised:2014-05-05 Online:2014-06-20 Published:2014-06-20
  • Contact: CHEN Huan-jiao

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摘要： 通过环境压力筛选获得了一株耐受30 g/L丙酸的产酸丙酸杆菌菌株(Propionibacterium acidipropionici WY320)，降低了发酵过程中丙酸的反馈抑制作用，采用正交实验设计优化了发酵培养基. 结果表明，发酵培养基最适的玉米浆、酵母膏和(NH4)2SO4浓度分别为60, 10和7.5 g/L. 该条件下，摇瓶培养耐酸菌株的发酵周期为240 h，丙酸产量为49.35 g/L，较优化前提高72.98%，产率为0.21 g/(L×h); 5 L发酵罐培养的发酵周期为168 h，丙酸产量达51.96 g/L，产率为0.31 g/(L×h)，较摇瓶培养提高47.62%，优于文献报道水平.

关键词: 产酸丙酸杆菌, 丙酸, 菌种选育, 正交设计, 发酵

Abstract: A propionic acid-resistant strain Propionibacterium acidipropionici WY320, which could tolerate up to 30 g/L of propionic acid, was obtained by environmental stress screening to reduce the feedback inhibition of propionic acid. Its fermentation medium was optimized by orthogonal experiments to improve the propionic acid yield. The results show that the optimal concentrations of corn steep powder, yeast extract and (NH4)2SO4 were 60, 10 and 7.5 g/L, respectively. The propionic acid production by the propionic acid-resistant strain could reach 49.35 g/L, which had a significant improvement of 72.98%, with a productivity of 0.21 g/(L×h). The scale-up experiment was carried out in a 5-L fermentor. The maximum propionic acid concentration of 51.96 g/L was obtained, and the productivity of propionic acid achieved to 0.31 g/(L×h), increased by 47.62% compared with the result of shaking flask experiment.

Key words: Propionibacterium acidipropionici, propionic acid, strain screening, orthogonal design, fermentation

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