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›› 2014, Vol. 14 ›› Issue (6): 1005-1009.

• 生化工程专栏 • 上一篇    下一篇

废弃棉织物浓磷酸预处理条件与高固酶水解基质浓度的关系

蔡郡倬 董祝君 邱婧雯 史宇 沈飞   

  1. 四川农业大学资源环境学院 四川农业大学资源环境学院 四川农业大学资源环境学院 四川农业大学资源环境学院 四川农业大学资源环境学院
  • 收稿日期:2014-09-29 修回日期:2014-11-13 出版日期:2014-12-20 发布日期:2014-12-20
  • 通讯作者: 蔡郡倬

Relationships between Concentrated H3PO4 Pretreatment Conditions and Substrate Concentration in High Solid Enzymatic Hydrolysis of Pretreated Waste Cotton-based Textiles

CAI Jun-zhuo DONG Zhu-jun QIU Jing-wen SHI Yu SHEN Fei   

  1. College of Resource and Environment, Sichuan Agricultural University College of Resource and Environment, Sichuan Agricultural University College of Resource and Environment, Sichuan Agricultural University College of Resource and Environment, Sichuan Agricultural University College of Resource and Environment, Sichuan Agricultural University
  • Received:2014-09-29 Revised:2014-11-13 Online:2014-12-20 Published:2014-12-20
  • Contact: CAI Jun-zhuo

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被引次数

3

摘要： 以浓磷酸预处理废弃棉织物，获得再生纤维素，对其进行酶水解，研究了预处理温度、时间和磷酸浓度与高固酶水解基质浓度的关系. 结果表明，温度和时间与高固酶水解潜力基质浓度均呈线性正相关性，温度从30℃提高至60℃，潜力基质浓度由4.6%提高至18.0%；时间由3.0 h延长至12.0 h，潜力基质浓度由10.8 %提高25.1%，但预处理时间过长不利于提高酶水解基质浓度. 磷酸浓度为85.0%时，潜力基质浓度达17.6%，改变浓度对潜力基质浓度提高影响较小. 废弃棉织物在60℃下用85.0%磷酸处理12.0 h后，高固酶水解的潜力基质浓度可达25.1%.

关键词: 废弃棉织物, 浓磷酸, 预处理, 高固酶水解, 潜力基质浓度

Abstract: In order to seek an efficient utilization way for waste cotton-based textiles, concentrated H3PO4 pretreatment was employed to regenerate cellulose for glucose production by enzymatic hydrolysis. The relationships of pretreatment temperature, time and H3PO4 concentration with substrate concentration for high solid enzymatic hydrolysis were investigated. The results indicated that the positive linear relationships existed in the pretreatment temperature, time and the substrate concentration. Based on glucose conversion of 70.0% as a criterion for high efficient hydrolysis, the potential substrate concentration could be improved from 4.6% to 18.0% as temperature was promoted from 30℃ to 60℃. When pretreatment time was prolonged from 3.0 to 12.0 h, the potential substrate concentration was correspondingly enhanced from 10.8% to 25.1%. However, excessive pretreatment time was not beneficial to the enhancement of potential substrate concentration. H3PO4 concentration could not greatly improve the potential substrate consistency in the range of 77.5%~82.5% except that 17.6% substrate concentration could be achieved at 85%. As a result, the maximum potential substrate concentration of 25.1% was achieved as the waste cotton-based textiles were pretreated at 60℃ with 85.0% H3PO4 for 12.0 h.

Key words: waste cotton-based textile, concentrated H3PO4, pretreatment, high solid enzymatic hydrolysis, potential concentration

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