Preparation and Characterization of PLA Nanoparticles
XU Jing WANG Lian-yan, YANG Ting-yuan LIU Yan-hui MA Guang-hui
College of Life Science and Technology, Beijing University of Chemical Technology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences College of Life Science and Technology, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：A modified precipitation method was used to prepare poly(lactic acid) (PLA) nanoparticles, and the response surface method applied to optimize the preparation parameters. The results showed that there were three main factors affecting the formation of nanoparticles in the preparation process, amount of PLA, volume fraction of ethanol in oil phase and total volume of oil phase. The mathematical model was established using the response surface methodology, and the optimal conditions were obtained with the aim of minimum average particle size, which were the amount of PLA at 76.82 mg, volume fraction of ethanol in oil phase 40%, and oil phase volume 19.49 mL. The size of PLA nanoparticles prepared under the optimal conditions was 109.1 nm and the PDI value 0.084, which was closer to the predicted value. The PLA nanoparticles were spherical solid particles with good dispersibility and uniform size distribution. The PLA nanoparticles could be phagocytosed by macrophage, and used in later immune experiments.
徐婧 王连艳 杨婷媛 刘艳辉 马光辉. 聚乳酸纳米颗粒的制备与表征[J]. 过程工程学报, 2015, 15(3): 495-500.
XU Jing WANG Lian-yan; YANG Ting-yuan LIU Yan-hui MA Guang-hui. Preparation and Characterization of PLA Nanoparticles. Chin. J. Process Eng., 2015, 15(3): 495-500.
2015, Vol. 15 
