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过程工程学报 ›› 2015, Vol. 15 ›› Issue (3): 495-500.DOI: 10.12034/j.issn.1009-606X.215132

• 生化工程专栏 • 上一篇    下一篇

聚乳酸纳米颗粒的制备与表征

徐婧 王连艳 杨婷媛 刘艳辉 马光辉   

  1. 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2015-02-02 修回日期:2015-04-15 出版日期:2015-06-20 发布日期:2015-06-20
  • 通讯作者: 徐婧

Preparation and Characterization of PLA Nanoparticles

XU Jing WANG Lian-yan, YANG Ting-yuan LIU Yan-hui MA Guang-hui   

  1. College of Life Science and Technology, Beijing University of Chemical Technology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences College of Life Science and Technology, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
  • Received:2015-02-02 Revised:2015-04-15 Online:2015-06-20 Published:2015-06-20
  • Contact: XU Jing

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摘要： 采用改良沉淀法制备了聚乳酸(PLA)纳米颗粒，采用响应面分析法建立数学模型对工艺条件进行优化. 结果表明，影响PLA颗粒制备的主要因素有PLA质量、油相中乙醇含量和油相体积，以平均粒径最小为目标，得到最优工艺条件为：聚乳酸质量76.82 mg，油相中乙醇含量40%(j)，油相体积19.49 mL. 按最优工艺条件所制PLA颗粒的粒径为109.1 nm，多分散系数PDI值为0.084，与预测值偏差较小. PLA纳米颗粒为球形实心颗粒，分散性良好且粒径均一. PLA纳米颗粒能被巨噬细胞摄取，有望作为免疫佐剂.

关键词: 聚乳酸, 纳米颗粒, 沉淀, 响应面法, 优化, 免疫

Abstract: A modified precipitation method was used to prepare poly(lactic acid) (PLA) nanoparticles, and the response surface method applied to optimize the preparation parameters. The results showed that there were three main factors affecting the formation of nanoparticles in the preparation process, amount of PLA, volume fraction of ethanol in oil phase and total volume of oil phase. The mathematical model was established using the response surface methodology, and the optimal conditions were obtained with the aim of minimum average particle size, which were the amount of PLA at 76.82 mg, volume fraction of ethanol in oil phase 40%, and oil phase volume 19.49 mL. The size of PLA nanoparticles prepared under the optimal conditions was 109.1 nm and the PDI value 0.084, which was closer to the predicted value. The PLA nanoparticles were spherical solid particles with good dispersibility and uniform size distribution. The PLA nanoparticles could be phagocytosed by macrophage, and used in later immune experiments.

Key words: poly(lactic acid), nanoparticles, precipitation, response surface methodology, optimization, immunization

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