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›› 2003, Vol. 3 ›› Issue (3): 0-0.

• 3 •    

转基因聚球藻7942培养中光传递及其对细胞生长的影响

朱笃，李元广，叶勤，魏晓东，章军   

  1. 1. 华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室，上海 200237；2. 厦门大学生命科学学院，福建 厦门 361005
  • 出版日期:2003-06-20 发布日期:2003-06-20

Light Transfer and Its Effect on Cell Growth in the Culture of Transgenic Synechococcus sp. PCC7942

ZHU Du1, LI Yuan-guang1, YE Qin1, WEI Xiao-dong1, ZHANG Jun2   

  1. Institute of Process Engineering, Chinese Academy of Sciences，Beijing 100080, China
  • Online:2003-06-20 Published:2003-06-20

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被引次数

12

摘要： 对转人源胸腺素a1基因聚球藻7942光生物反应器分批培养过程中光的分布、平均光强变化及其对藻细胞生长的影响进行了分析. 结果表明，Hyperbolic光衰减模型较之Lambert-Beer光衰减模型能更好地描述转基因聚球藻7942培养液中的光衰减；随着藻细胞密度的增加，光生物反应器内平均光强不断减小，藻细胞的光能比吸收速率也不断降低，反应器中“暗区”体积不断加大；培养时间0~1.5 d，藻细胞能获得充足光能，因而呈指数生长；1.5 d后藻细胞处于光限制，比生长速率不断下降，但体积光能吸收速率达到最大并保持恒定，藻细胞进入线性生长期；5 d后光生物反应器“暗区”体积超过了反应器总体积的50%，藻细胞吸收的光能用于维持的比例不断增加，从而导致生长速率下降；培养8 d，转基因聚球藻7942中人源胸腺素a1表达量为4.53 mg/L.

关键词: 光衰减, 光分布, 平均光强, 分批培养, 光生物反应器, 人胸腺素a1, 转基因聚球藻7942

Abstract: A gold leaching process by using in situ oxidation products of added elemental sulfur in Ca(OH)2 solution was investigated. A gold concentrate containing 45 g/t Au was tested and 85%~87% of gold were leached. The leached gold depends mainly on the initial molar ratio of elemental sulfur to the hydroxyl ion, the consumption of oxygen and the reaction temperature. Adding some surfactants, such as lignosulfonic calcium, at lower concentration increased the leached Au but at higher concentration decreased it. Both of thermodynamic analysis and experimental results show that thiosulfate is the major complexing agent for gold in the process.

Key words: light attenuation, light distribution, average irradiance, batch culture, photobioreactor, human thymosin a1, transgenic Synechococcus sp. PCC7942

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