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›› 2002, Vol. 2 ›› Issue (6): 0-0.

• 6 •    

铅、镉和锌污染对芦苇幼苗氧化胁迫和抗氧化能力的影响

王正秋，江行玉，王长海   

  1. 1. 烟台大学海洋生化工程研究所，山东 烟台 264005； 2. 山东农业大学农学院，山东 泰安 271018
  • 出版日期:2002-12-20 发布日期:2002-12-20

Effects of Pb, Cd, and Zn on Oxidative Stress and Antioxidative Ability in Phragmites australis

WANG Zheng-qiu1, JIANG Xing-yu2, WANG Chang-hai1   

  1. 1. Institute of Marine Biochemical Engineering, Yantai University, Yantai, Shandong 264005, China; 2. Agricultural College, Shandong Agricultural University, Tai￠an, Shandong 271018, China
  • Online:2002-12-20 Published:2002-12-20

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被引次数

337

摘要： 对Pb, Cd和Zn胁迫下芦苇幼苗叶片和根内超氧阴离子自由基(O2-)和过氧化产物丙二醛(MDA)的含量、电解质渗漏以及超氧化物岐化酶(SOD)和过氧化物酶(POD)活性进行了研究. 结果表明，受3种重金属的影响，叶片和根内O2- 积累，MDA含量增加，伴随着电解质渗漏增大，显示发生了膜脂过氧化，细胞膜系统遭到破坏；作为植物抗氧化系统中的关键酶，SOD和POD活性高于对照，说明在重金属胁迫下芦苇幼苗体内的抗氧化能力增强. 可见，在重金属污染下细胞内O2- 浓度升高带来的膜脂过氧化增强是重金属伤害植物的主要原因；而保护酶系统SOD和POD活性的升高则可能是芦苇抗过氧化的机理之一.

关键词: 芦苇, 铅, 镉, 锌, 氧化胁迫, 抗氧化能力

Abstract: Superoxide anion radical, MDA, electrolyte leakage, as well as activities of SOD and POD in the leaf and root cells of Phragmite australis were investigated under the conditions of Pb, Cd, and Zn stresses. Under these conditions, the accumulation of superoxide anion radical and MDA, and the electrolyte leakage of the cell membrane increased in the plant cells, which indicated that the peroxidation of cell membrane lipid was induced and the membrane system was damaged. Higher activity of SOD and POD, two key enzymes of the antioxidative system, was observed in all Pb, Cd, and Zn treated plant cells. These results suggest that the enhancement of peroxidation of membrane lipid induced by superoxide anion radical may be one of the heavy metal injury mechanisms in P. asutralis. Furthermore, the accumulation of the two enzymes would be part of general defense strategies to cope with oxidative stress.

Key words: Phragmites australis, Pb, Cd, Zn, oxidative stress, antioxidative ability

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