Abstract：The cultivation of protocorm-like bodies of Dendrbium huoshanense was carried out in a 10 L air-lift bioreactor. The effects of inoculum size, aeration rate, carbon, phosphate sources on the cell growth and accumulation of polysaccharides were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L (fresh weight) cells and aeration rate of 0.5 L/min. The optimal concentrations of sucrose and phosphate in the medium for the cell growth were 30 g/L and 2.5 mmol/L, respectively. A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides. The results in two-step cultivation indicated that the maximum biomass and polysaccharides yield were 46.7 g/L (dry weight) and 8.15 g/L, which are 1.5 and 2.6 fold of those in one-step cultivation respectively.
2007, Vol. 7 
