葡萄糖和木糖双底物生物合成2,3-丁二醇的条件优化

›› 2009, Vol. 9 ›› Issue (6): 1174-1177.

葡萄糖和木糖双底物生物合成2,3-丁二醇的条件优化

张根林邓辉鲁建江孙守玲李春

石河子大学化学化工学院石河子大学化学化工学院石河子大学化学化工学院石河子大学化学化工学院北京理工大学生命科学与技术学院生物技术系

收稿日期:2009-06-30 修回日期:2009-09-28 出版日期:2009-12-20 发布日期:2009-12-20
通讯作者: 张根林

Optimization of Cofermentation of Glucose and Xylose for Sythesis of 2,3-Butanediol

ZHANG Gen-lin DENG Hui LU Jian-jiang SUN Shou-ling LI Chun

School of Chemistry and Chemical engineering, Shihezi Universit School of Chemistry and Chemical engineering, Shihezi Universit School of Chemistry and Chemical engineering, Shihezi Universit School of Chemistry and Chemical engineering, Shihezi Universit Department of Biotechnology, School of Life Science and Technology, Beijing Institute of Technology

Received:2009-06-30 Revised:2009-09-28 Online:2009-12-20 Published:2009-12-20
Contact: ZHANG Gen-lin

摘要/Abstract

摘要： 针对利用葡萄糖和木糖合成2,3-丁二醇的Klebsiella pneumoniae XJ-Li菌，优化培养基组成与发酵条件，围绕五、六碳糖共代谢的特点，探讨简单可行的代谢调控方法. 结果表明，60 g/L葡萄糖和40 g/L木糖为碳源，5.75 g/L NH4H2PO4为氮源，pH值维持在5.5，培养温度38℃, 2,3-丁二醇浓度可达19.24 g/L. 确定了pH值调控和外源添加维生素C的调控方式，通过调节发酵过程中pH值于5.5左右，使2,3-丁二醇的产量提高了16.4%；添加60 mg/L维生素C调节培养基的氧化还原状态，可使2,3-丁二醇的产量提高44.3%，批式发酵48 h, 2,3-丁二醇终浓度可达33.47 g/L.

关键词: 3-丁二醇, 葡萄糖, 木糖, Klebsiella pneumoniae

Abstract: The production conditions of 2,3-butanediol by Klebsiella pneumoniae XJ-Li from glucose and xylose were optimized, and the regulation methods for 2,3-butanediol fermentation were also determined based on the metabolic features of hexose and pentose. The results displayed that suitable culture conditions were defined as glucose of 60 g/L, xylose of 40 g/L, ammonium dihydrogen phosphate of 5.75 g/L and pH of 5.5, and the concentration of 2,3-butanediol reached 19.24 g/L under the conditions when the strain was cultured at 38℃. The yield of 2,3-butanediol was increased by 16.4% after maintaining the pH about 5.5 in the fermentation process. And 60 mg/L of vitamin C was added into medium to regulate the redox state of system, which made the yield of 2,3-butanediol improved by 44.3% of 2,3-butanediol fermentation. 33.47 g/L of 2,3-butanediol was obtained in batch fermentation for 48 h under optimum culture conditions.

Key words: 3-butanediol, glucose, xylose, Klebsiella pneumoniae

中图分类号:

TQ923

张根林邓辉鲁建江孙守玲李春. 葡萄糖和木糖双底物生物合成2,3-丁二醇的条件优化[J]. , 2009, 9(6): 1174-1177.

ZHANG Gen-lin DENG Hui LU Jian-jiang SUN Shou-ling LI Chun. Optimization of Cofermentation of Glucose and Xylose for Sythesis of 2,3-Butanediol[J]. , 2009, 9(6): 1174-1177.

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