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›› 2008, Vol. 8 ›› Issue (3): 561-566.

• 生化工程专栏 • 上一篇    下一篇

SAM产生菌酿酒酵母HYS98的发酵方式

沈剑 李元广 沈国敏 杨东   

  1. 华东理工大学生物反应器工程国家重点实验室 华东理工大学海洋生化工程研究所 生物反应器工程国家重点实验室, 华东理工大学海洋生化工程研究所,生物反应器工程国家重点实验室 上海泽元海洋生物技术有限公司
  • 收稿日期:2007-11-09 修回日期:2008-03-31 出版日期:2008-06-20 发布日期:2008-06-20
  • 通讯作者: 李元广

Fermentation Modes of Saccharomyces cerevisiae HYS98 for High Productivity of S-adenosyl-L-methionine

SHEN Jian LI Yuan-guang SHEN Guo-min YANG Dong   

  1. Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Inst.Marine Bioprocess Eng., East China Univ. Sci. Technol Inst. Marine Bioprocess Eng., State Key Lab. Bioreact. Eng., East China Univ. Sci. Technol. Shanghai Zeyuan Marine Biotechnology Ltd
  • Received:2007-11-09 Revised:2008-03-31 Online:2008-06-20 Published:2008-06-20
  • Contact: LI Yuan-guang

摘要: 分别研究了分批发酵、恒速流加发酵、变速流加发酵和分批补料发酵4种操作方式下酿酒酵母HYS98发酵生产SAM的代谢特征,并考察了不同发酵方式对发酵结果的影响. 结果表明,酿酒酵母HYS98发酵生产SAM的最佳发酵模式为分批补料发酵,SAM的平均产率和菌体生长速率分别达0.123和1.030 g/(L×h),比国内外文献报道的最高水平分别提高了68%和73%,为发酵工艺进一步优化奠定了基础.

关键词: 酿酒酵母HYS98, S-腺苷甲硫氨酸, 发酵方式

Abstract: The metabolic characteristics under four operation ways of batch fermentation, constant feeding rate fermentation, changing feeding rate fermentation and batch feeding fermentation, were studied in a 5 L fermentator. And the influences of different S-adenosyl-L-methionine (SAM) fermentation modes were investigated. The optimum fermentation mode among them of Saccharomyces cerevisiae HYS98 was batch feeding. The average productivity and cell growth rate of the feed batch fermentation were obtained as 0.123 g/(L×h) and 1.030 g/(L×h), which were 68% and 73% higher than the highest levels reported in the literature respectively. The results laid the foundations for further optimization of SAM fermentation process.

Key words: Saccharomyces cerevisiae HYS98, S-adenosyl-L-methionine, fermentation mode