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›› 2011, Vol. 11 ›› Issue (3): 468-474.

• 生化工程专栏 • 上一篇    下一篇

螺旋藻水溶性多糖提取液的絮凝过程

王峰 顾志国 印小燕   

  1. 江南大学化工学院 江南大学化工学院 江南大学化工学院
  • 收稿日期:2011-01-12 修回日期:2011-03-24 出版日期:2011-06-20 发布日期:2011-06-20
  • 通讯作者: 王峰

Flocculation Process during Extraction of Water-soluble Polysaccharides from Spirulina

WANG Feng GU Zhi-guo YIN Xiao-yan   

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences
  • Received:2011-01-12 Revised:2011-03-24 Online:2011-06-20 Published:2011-06-20
  • Contact: WANG Feng

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摘要： 螺旋藻热水粗提取液中含大量表面带负电荷的微粒. 根据静电中和絮凝机理，用阳离子絮凝剂聚合硫酸铁(PFS)处理粗提液的絮凝率为80.32%，脱色率75.48%，蛋白质去除率50.30%，总糖保存率85.40%，絮凝效果不受粗提取液pH值、絮凝时间和温度的影响. 提取液在絮凝后离心去杂，再经乙醇沉淀，多糖沉淀用去离子水复溶，所得复溶液中总固形物的总糖含量达86.0%. Zeta电势显示，PFS在酸性和中性条件下能完全中和颗粒表面电荷，碱性条件下能大幅度降低颗粒表面电荷. 絮体形态显示，添加PFS促进了提取液中微粒的团聚，提高了微粒的可沉降性.

关键词: 藻多糖提取液, 絮凝过程, 静电中和, Zeta电势, 絮体形态

Abstract: A large quantity of small negatively charged particles exists in the hot water extraction from Spirulina. According to the mechanism of electrostatic neutralization, the cationic flocculants were applied in the treatment of the hot water extraction. The desirable flocculation rate, decoloration rate, protein removal rate and retention rate of total sugar obtained with the addition of PFS were 80.32%, 75.48%, 50.30%, 85.40% respectively. The variation of pH value of extract, time and temperature of operation did not influence the flocculation. Furthermore, the solution collected after flocculation was treated with alcohol, and the precipitate redissolved in water. The total sugar of total solid in the redissolved solution was 86.0%. Zeta potential of particles in the extract revealed that PFS could neutralize the surface potential of particles under acidic and neutral conditions, reduce the surface potential significantly under alkaline condition. The morphology displayed that the agglomeration of small particles in extract was promoted by the addition of PFS and the settleability of particles was improved.

Key words: polysaccharide extraction, flocculation proces, electrostatic neutralization, Zeta potential, morphology

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