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›› 2007, Vol. 7 ›› Issue (5): 1009-1013.

• 生化工程专栏 • 上一篇    下一篇

硫酸盐还原菌的固定化

曹俊雅,张广积,毛在砂,方兆珩,韩宝玲   

  1. 中国科学院过程工程研究所
  • 出版日期:2007-10-20 发布日期:2007-10-20

Immobilization of Sulfate Reducing Bacteria

CAO Jun-ya,ZHANG Guang-ji,MAO Zai-sha,FANG Zhao-heng,HAN Bao-ling   

  1. Institute of Process Engineering, CAS
  • Online:2007-10-20 Published:2007-10-20

摘要： 针对低浓度生物冶金浸出液难以处理的现状，利用硫酸盐还原菌(Sulfate Reducing Bacteria, SRB)沉淀分离有价金属离子的微生物法是很有应用前景的处理方法. 本工作以提高SRB的还原活性为出发点，通过间歇式实验首先确定适宜的初始COD/SO42-比值和pH值，进而探讨固定化载体对SRB还原性能的影响. 研究结果表明，初始COD/SO42-比值为3、初始pH值为7时SRB具有较高的生物活性. 影响生物膜形成及SRB活性的首要因素是固定化载体的表面粗糙度，其次是孔容. 表面光滑的惰性玻璃珠作为载体时没有生物膜形成，SRB的活性低，SO42-的去除率仅为50%；其他载体均观测到生物膜，且载体的孔容越大，SRB的SO42-去除率也越高. 从SO42-的去除效果和工艺的稳定性考虑，多孔聚氨酯泡沫是较优的固定化载体，SRB的还原活性高，SO42-的去除率高达95%，工艺操作简便.

关键词: 硫酸盐还原菌, 固定化载体, COD/SO42-比值, pH值

Abstract: In view of the difficulty in treating the bioleaching solutions, the selective precipitation of metals using H2S produced biologically by sulfate reducing bacteria (SRB) was as an alternative process. At first, the initial COD/SO42- ratio and pH value were determined, and then the capability of different immobilized carriers was investigated by batch experiments. The results showed that high removal rate of SO42- was achieved when the initial COD/SO42- ratio was 3 and the initial pH value was 7. In the experiments, it was found that two factors were important for influencing the formation of biofilm and the reducing capability of SRB, the roughness of the immobilized carriers and the pore volume. There was no biofilm found when glass beads were immobilized carrier, and the reducing capability of SRB was low, the removal rate of SO42- was only 50%. Furthermore, the removal rate of SO42- increased with the pore volume of immobilized carriers. So, in view of the stability of process and the removal rate of SO42-, polyurethane foam was top priority as the immobilized carrier in the experiments, the removal rate of SO42- with it reached 95%, and the process was easily operated.

Key words: sulfate reducing bacteria, immobilized carriers, COD/SO42- ratio, pH