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›› 2002, Vol. 2 ›› Issue (3): 0-0.

• 3 •    

绿色化工新例—利用基因重组蓝细菌生产PHB的初步结果

吴桂芳,沈忠耀   

  1. 清华大学化工系生物化工研究所，北京 100084
  • 出版日期:2002-06-20 发布日期:2002-06-20

Effect of Preparation Methods on the Properties of Zr0.9Ti0.1(Ni,Co,Mn,V)2+a Hydrogen Storage Alloys

ZHANG Guo-fan1, WEN Ming-fen1, ZHAI Yu-chun1, MA Rong-jun1, CHEN Lian2, TONG Min2   

  1. Dept. Chemical Engineering, Biochemical Engineering Institute, Tsinghua University, Beijing 100084, China
  • Online:2002-06-20 Published:2002-06-20

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摘要： 在发现蓝细菌糖原合成与PHB合成相竞争的基础上，提出用基因工程手段改造蓝细菌，使催化糖原合成的关键酶?ADP-葡萄糖焦磷酸羧化酶的agp基因部分为红霉素抗性基因所取代，从而将与PHB合成形成竞争反应的糖原合成途径删除. 对该突变株的构建过程及培养进行了初步研究，所得突变株生长速度提高，最终生物量较野生株高，表明突变株细胞的光合效率较野生株细胞提高. 在突变株体内未检测到糖原，光合自养时PHB含量由野生株的3.4%提高到约15%.

关键词: 绿色化工, 蓝细菌, PHB, 基因重组

Abstract: Based on the understanding that the cyanobacterial glycogen biosynthesis is a competitive pathway to cyanobacterial PHB biosynthesis, a genetically engineered cyanobacterium was constructed by partially replacing the agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis by an erythromycin resistance cassette. The obtained mutant propagated faster and the final biomass was higher against its wild-type species, which indicated a much higher photosynthetic efficiency in the mutant. No glycogen was detected in the mutant. Under the photoautotrophic condition, the intracellular PHB content was improved to 15% of the dry weight as compared to 3.4% in the wild-type strain.

Key words: hydrogen storage alloy, spinning, enthalpy, discharge capacity

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