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›› 2008, Vol. 8 ›› Issue (4): 794-797.

• 生化工程专栏 • 上一篇    下一篇

少根根霉利用木糖和葡萄糖分步发酵制备富马酸

刘宁 李霜 何皓 吴华 黄和 嵇松杨   

  1. 山东理工大学 南京工业大学 制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 北京盈信阳光生物技术有限公司
  • 收稿日期:2008-03-27 修回日期:2008-06-12 出版日期:2008-08-20 发布日期:2008-08-20
  • 通讯作者: 黄和

Stepped Utilization of Xylose and Glucose in Fermentation of Fumaric Acid by Rhizopus arrhizus

LIU Ning XU Qing HE Hao WU Hua HUANG He JI Song-yang   

  1. College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology WinHonor Bioscience Limited Corporation
  • Received:2008-03-27 Revised:2008-06-12 Online:2008-08-20 Published:2008-08-20
  • Contact: HUANG He

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被引次数

29

摘要： 将木糖与葡萄糖分别用于少根根霉种子培养及发酵产富马酸2个阶段，少根根霉利用木糖进行种子培养时，最适木糖浓度为30 g/L，摇瓶最适孢子浓度为(4~6)×105 mL-1，最佳种龄32~40 h，最适接种量为8%(j)，在含有100 g/L葡萄糖的产酸发酵培养基中发酵72 h后，富马酸最高浓度达53.51 g/L. 研究结果表明，可以利用木糖替代葡萄糖进行少根根霉的种子培养，分步发酵制备富马酸.

关键词: 富马酸, 木糖, 葡萄糖, 少根根霉, 分步发酵

Abstract: The fumaric acid production by Rhizopus arrhizus using xylose as the carbon source of seed culture medium and glucose as carbon source of fermentation medium was studied. In the seed culture, the biomass of Rhizopus arrhizus obtained using xylose was more than that using glucose. The conditions of the seed culture were optimized. The results showed that the optimal xylose concentration was 30 g/L, the suitable spore concentration range (4~6)×105 mL-1 and the seed age range 32~40 h. The final concentration of fumaric acid was 53.51 g/L in the fermentation medium containing 100 g/L glucose. The result implies that the problem of low conversion rate of xylose could be avoided, and the availability of xylose improved, which would facilitate further study on the stepped utilization of lignocellulose hydrolysate in fermentation of fumaric acid.

Key words: fumaric acid, xylose, glucose, Rhizopus arrhizus, stepped utilization

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