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›› 2008, Vol. 8 ›› Issue (4): 798-801.

• 生化工程专栏 • 上一篇    下一篇

微水相中S-2-苯基丙酸的制备

张波 曾虹燕 王亚举 夏葵 曾亮 黄炎   

  1. 湘潭大学化工学院生物技术研究所 湖南湘潭大学化工学院 湖南湘潭大学化工学院 湖南湘潭大学化工学院 爱斯特(成都)医药技术有限公司 湖南湘潭大学化工学院
  • 收稿日期:2007-10-26 修回日期:2008-04-25 出版日期:2008-08-20 发布日期:2008-08-20
  • 通讯作者: 曾虹燕

Preparation of S-2-Phenylpropionic Acid in Micro-aqueous Phase

ZHANG Bo ZENG Hong-yan WANG Ya-ju XIA Kun ZENG Liang HUANG Yan   

  1. Biotechnology Institute ,Chemical Engineering College of Xiangtan University Biothchnology Research Institute, Xiangtan University Biothchnology Research Institute, Xiangtan University Biothchnology Research Institute, Xiangtan University AstsTech (Chengdu) Pharmaceutical Co. Ltd Biothchnology Research Institute, Xiangtan University
  • Received:2007-10-26 Revised:2008-04-25 Online:2008-08-20 Published:2008-08-20
  • Contact: ZENG Hong-yan

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被引次数

13

摘要： 以外消旋体2-苯基丙酸为原料，探讨不同醇对2-苯基丙酸酯化反应的影响，确定异丙醇为最佳醇底物. 通过微水相优化拆分制备S-2-苯基丙酸的反应条件，确定了青霉素酰化酶拆分制备S-2-苯基丙酸的最佳工艺参数. 实验结果表明，95%四氢呋喃水溶液中，酶浓度1.5 mg/mL，底物浓度0.3 g/mL，30℃，pH 7为最佳酶拆分反应条件，S-2-苯基丙酸的收率为94.5%，ee值为97.0%.

关键词: 2-苯基丙酸, 酶拆分, 青霉素酰化酶, 微水相

Abstract: A new bio-enzymatic catalysis procedure of producing enantiomeric S-2-phenylpropionic acid was investigated. The effect of different alcohols on the esterification of 2-phenylpropionic acid was performed and the optimal alcohol was isopropanol. S-2-phenylpropionic acid was obtained by hydrolysis of 2-phenylpropionic ester catalyzed by penicillin G acylase (PGA) in the micro-aqueous phase. In the 95% (j) tetrahydrofuran solvent system, the optimal conditions were obtained as follows: enzyme PGA at 1.5 mg/mL, 2-phenylpropionic ester 0.3 g/mL, 30℃, and pH 7. The maximum yield and ee value of S-2-phenylpropionic acid were 94.5% and above 97.0%, respectively.

Key words: 2-phenylpropionic acid, enzymatic resolution, penicillin G acylase, micro-aqueous phase

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