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›› 2009, Vol. 9 ›› Issue (2): 344-249.

• 生化工程专栏 • 上一篇    下一篇

微生物谷氨酰胺转胺酶对羊毛蛋白纤维改性作用的性能表征

崔莉 范雪荣 陈坚 堵国成 王平   

  1. 江南大学纺织服装学院 江南大学纺织服装学院 江南大学工业生物技术教育部重点实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2008-11-07 修回日期:2009-01-12 出版日期:2009-04-20 发布日期:2009-04-20
  • 通讯作者: 崔莉

Characterization of Wool Fibers Modified by Using Microbial Transglutaminse

CUI Li FAN Xue-rong CHEN Jian, DU GUo-cheng, WANG Ping   

  1. Key Lab Science and Technology of Eco-Textile, Ministry of Education, Southern Yangtze University Key Lab Science and Technology of Eco-Textile, Ministry of Education, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University Lab of Environmental Biotechnology, School of biotechnology Southern Yangtze University
  • Received:2008-11-07 Revised:2009-01-12 Online:2009-04-20 Published:2009-04-20
  • Contact: CUI Li

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摘要： 采用SEM, AFM, FT-IR及SDS-PAGE等技术表征了谷氨酰胺转胺酶(Tgase)对不同预处理的羊毛的改性效果. 结果表明，KMnO4预处理主要引起羊毛红外光谱S?O振动区(1000~1200 cm-1)变化，蛋白酶预处理及TGase的作用没有显著改变羊毛的红外光谱吸收. 与H2O2相比，KMnO4与蛋白酶使羊毛鳞片结构破坏，纤维表面的平均粗糙度由12.5 nm升高至24 nm，TGase的作用未明显改变纤维的表面形态. SDS-PAGE电泳分析显示TGase可催化羊毛蛋白间通过共价交联形成聚合物.

关键词: 谷氨酰胺转胺酶, 羊毛, 结构

Abstract: The modification mechanism of wool fibers after different pretreatments with microbial transglutaminase (TGase) was analyzed by SEM, AFM, FT-IR ATR and SDS-PAGE. The results showed that KMnO4 pretreatment induced the alteration of characteristic peaks at around 1000~1200 cm-1 and the FT-IR absorption spectrum did not change obviously after protease pretreatment H2O2 and TGase treatment. The scale structure of wool surface was significantly damaged after KMnO4 and protease pretreatment in comparison with H2O2 pretreatment, which increased the average roughness of fiber surface from 12.5 to 24 nm. The surface morphology of fibers after TGase treatment was not evidently altered, but SDS-PAGE spectrum demonstrated that new biopolymers with higher molecular weight from wool keratin were formed by using TGase.

Key words: transglutaminase, wool, structure

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