关键词: 杂色云芝, 酶活性, 降解, 结构

Abstract: Lignin biodegradation has important significance for environmental protection and resource usage. In the present work, Coriolus versicolor was cultivated in the solid media of rice straw supplemented with glucose and ammonium chloride. The activities of lignocellulolytic enzymes and the contents of main chemical constituents were determined during the process of culturing. The straw structure was determined through scanning electron microscope and Fourier-transform infrared spectroscopy. The results show that enzyme activity is affected by concentrations of carbon and nitrogen sources. The most suitable condition for production of cellulases, lignin peroxidases, manganese-dependent peroxidases, laccases is high carbon and low nitrogen (glucose 2%, ammonium chloride 0.03%), while the one of hemi-cellulases is low carbon and low nitrogen (glucose 0.15%, ammonium chloride 0.03%). The emzymes have crucial effect on the biodegradation, and the rate of biodegradation is directly influenced by the enzyme activities. Biodegradation of cellulose and hemi-cellulose significantly accelerates the biodegradation of lignin. 65.39% of cellulose, 45.11% of hemi-cellulose and 39.16% of lignin in rice straw are biodegradated after 32 d cultivation. Structure of rice straw has changed evidently after biodegradation.

Key words: Coriolus versicolor, enzyme activities, biodegradation, structure