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›› 2004, Vol. 4 ›› Issue (2): 182-186.

• 绿色化学与技术专栏 • 上一篇    下一篇

姬松茸发酵条件及其菌丝水提物抗黄瓜黑星病的研究

王晓谊,齐鸿雁,张洪勋   

  1. 中国科学院生态环境研究中心
  • 出版日期:2004-04-20 发布日期:2004-04-20

Investigation on Cucumber Scab Resistance with Mycelial Water Extracts from Agaricus blazei Murrill Fermentation

WANG Xiao-yi,QI Hong-yan,ZHANG Hong-xun   

  1. 中国科学院生态环境研究中心
  • Online:2004-04-20 Published:2004-04-20

摘要: 对姬松茸(Agaricus blazei Murrill)发酵条件及其菌丝水提物对黄瓜黑星病的诱导抗病性进行了研究. 为得到菌丝发酵的最大生物量,通过正交试验确定了姬松茸的液体发酵培养基配方为(%):玉米淀粉3.50,酵母粉0.30, KH2PO4 0.20, MgSO4×7H2O 0.15, CaCl2 0.05, 玉米浆0.2. 于25oC发酵8 d,生物量达到8.65 g/L. 经黄化子叶喷雾接种法测定,姬松茸菌丝水体物对黄瓜黑星病的平均诱导抗病效果为74.04%. 毒性测定结果表明,该产物按农药分级属于低毒. 证明了姬松茸菌丝水提物是一种高效低毒的生物农药.

关键词: 姬松茸, 菌丝水提物, 诱导抗病性

Abstract: The optimal formula of fermentation medium obtained by orthogonal experiment were as follows (%): maize amylum 3.50, yeast extract 0.30, KH2PO4 0.20, MgSO4×7H2O 0.15, CaCl2 0.05 and maize plasm 0.20. By the culture at 25oC for 8 d, the maximum mycelial biomass reached 8.65 g/L. The average resistance effect of the mycelial water extracts to the disease of cucumber scab reached 74.04% by spraying spore suspension on yellow cotyledon. The toxicity test showed that the mycelial water extract is a low toxic according to pesticide classification. The mycelial water extract is proved to be a high-effect and low-toxicity bio-pesticide.

Key words: Agaricus blazei Murrill, mycelial water extracts, induced disease resistance