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›› 2004, Vol. 4 ›› Issue (1): 37-41.

• 生化工程专栏 • 上一篇    下一篇

酶法合成月桂酸单甘油酯

马清仪,陈洪,杜毅,许平   

  1. 山东大学微生物技术国家重点实验室
  • 出版日期:2004-02-20 发布日期:2004-02-20

Enzymatic Synthesis of Decanoic Acid Monoglycerides

MA Qing-yi,CHEN Hong,DU Yi,XU Ping   

  1. 山东大学微生物技术国家重点实验室
  • Online:2004-02-20 Published:2004-02-20

PDF

被引次数

35

摘要： 采用脂肪酶直接对月桂酸和甘油进行酯化，考察了反应温度、水份、反应物摩尔比及酶用量对月桂酸单甘油酯合成的影响，确定了酶促反应进行的最适反应温度为60℃，反应物摩尔比为1:1，酶用量为0.5%，最终产物含量可达70%以上，后处理过程大大简化.

关键词: 月桂酸单甘油酯, 脂肪酶, 酯化, 双底物反应

Abstract: Decanoic acid monoglyceride was synthesized by esterification of glycerol with decanoic acid. The effects of molar ratio of glycerol to fatty acid, reaction temperature, content of water and enzyme ratio over total weight on the reaction were studied. The optimum reaction conditions were confirmed to be 50oC, mole ratio of substrates 1:1, enzyme ratio over total weight 0.5%, and the purity of monoglyceride product in the reaction mixture was more than 70%. The advantage of the process includes simple procedure, high product content, and gentle reaction conditions.

Key words: decanoic acid, monoglycerides, lipase, esterification