关键词: 地衣芽孢杆菌, b-甘露聚糖酶, 代谢通量分析, 线性规划

Abstract: Intracellular metabolic activity of Bacillus licheniformis for synthesis of b-mannanase was studied based on metabolic flux analysis (MFA). A metabolic network of Bacillus licheniformis for synthesis of b-mannanase was established, and the metabolic mass balance based stoichiometric model of the above network was also built. The metabolic flux model was solved by using linear programming optimization based on the target functions of the metabolic flux distribution of maximum b-mannanase synthesis and maximum cell density. Furthermore, the maximum theoretical yield of b-mannanase from Bacillus licheniformis was calculated as 57.87%. Finally, five important metabolic nodes in the metabolic network of Bacillus licheniformis were analyzed, and two nodes, ribulose 5-phosphate and oxalacetate, were characterized as non-rigid ones, which would guide us to enhance the productivity of b-mannanase using gene engineering.

Key words: Bacillus licheniformis, b-mannanase, metabolic flux analysis, linear programming