Kitasatospora sp.MY 5-36菌株补料重复发酵生产ε-聚赖氨酸

›› 2010, Vol. 10 ›› Issue (2): 344-347.

Kitasatospora sp.MY 5-36菌株补料重复发酵生产ε-聚赖氨酸

张扬张全景冯小海徐虹

南京工业大学食品与轻工学院材料化学工程国家重点实验室南京工业大学食品与轻工学院材料化学工程国家重点实验室南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院

收稿日期:2009-11-10 修回日期:2010-01-11 出版日期:2010-04-20 发布日期:2010-04-20
通讯作者: 徐虹

Repeated Fermentation with Variable Feeding for Production of e-Poly-L-lysine by Kitasatospora sp. MY 5-36

ZHANG Yang, ZHANG Quan-jing, FENG Xiao-hai XU Hong

State Key Laboratory of Materials-oriented Chemical Engineering， College of Food Sciences and Light Industry， Nanjing University Of Technology State Key Laboratory of Materials-oriented Chemical Engineering， College of Food Sciences and Light Industry， Nanjing University Of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology

Received:2009-11-10 Revised:2010-01-11 Online:2010-04-20 Published:2010-04-20
Contact: XU Hong

摘要/Abstract

摘要： 以Kitasatospora sp. MY 5-36菌株为研究对象，首次采用带补料的重复发酵工艺发酵生产e-聚赖氨酸(e-PL)，并对工艺参数进行初步研究. 在摇瓶上对重复发酵e-PL的可行性及稳定性进行了验证，并在5 L发酵罐上初步构建了带补料的重复发酵e-PL工艺. 研究结果表明，重复发酵过程中最佳留种量为10%(j). 通过对间歇补料、变速补料2种补料方式的考察，确定当残糖浓度下降至10 g/L时，采用变速补料并控制残糖浓度在10 g/L时能较好地进行带补料的重复发酵生产e-PL. 经5批次发酵后发酵体系仍具有较高的e-PL生产能力，e-PL批次平均终浓度为20 g/L，生产强度达到0.159 g/(L×h).

关键词: e-聚赖氨酸, Kitasatospora sp. MY 5-36菌株, 重复发酵

Abstract: Repeated fermentation with variable feeding for production of e-poly-L-lysine (e-PL) by Kitasatospora sp. MY 5-36 was investigated. It was demonstrated that the repeated fermentation in shaking flask had good feasibility and stability. The e-PL production was also studied in a 5 L bioreactor. The maximum e-PL concentration was obtained when 10%(j) fermentation broth was left as the seed for the next batch of repeated fermentation. The feeding should be started when the fermentation broth of residual sugar concentration decreased to 10 g/L. During the repeated fermentation, the glucose concentration was maintained at about 10 g/L with variable flow feeding. A high level of e-PL production capacity still remained after 5 batches of fermentation, with approximate average batch e-PL concentration of 20.0 g/L under a productivity of 0.159 g/(L×h).

Key words: e-poly-L-lysine, Kitasatospora sp. MY 5-36, repeated fermentation

中图分类号:

TQ920

张扬张全景冯小海徐虹. Kitasatospora sp.MY 5-36菌株补料重复发酵生产ε-聚赖氨酸[J]. , 2010, 10(2): 344-347.

ZHANG Yang; ZHANG Quan-jing; FENG Xiao-hai XU Hong. Repeated Fermentation with Variable Feeding for Production of e-Poly-L-lysine by Kitasatospora sp. MY 5-36[J]. , 2010, 10(2): 344-347.