欢迎访问过程工程学报, 今天是

›› 2012, Vol. 12 ›› Issue (5): 839-843.

• 生化工程专栏 • 上一篇    下一篇

纤维素酶法提取黄芪多糖的动力学

魏凤玉 康家胜 张宇   

  1. 合肥工业大学化学工程学院 合肥工业大学化工学院 合肥工业大学化学工程学院
  • 收稿日期:2012-05-29 修回日期:2012-07-18 出版日期:2012-10-20 发布日期:2012-10-20
  • 通讯作者: 魏凤玉

Extraction Kinetics of Astragalus Polysaccharides by Cellulase-assisted Aqueous Extraction

WEI Feng-yu 康家胜 KANG Jia-Sheng ZHANG Yu   

  1. School of Chemical Engineering, HeFei University of Technology School of Chemical Engineering,HeFei University of Technology School of Chemical Engineering,HeFei University of Technology
  • Received:2012-05-29 Revised:2012-07-18 Online:2012-10-20 Published:2012-10-20
  • Contact: WEI Feng-yu

摘要: 采用纤维素酶辅助提取(CAE)黄芪多糖(APS),探讨了温度和粒径等对提取动力学特征的影响,采用扫描电镜观察了CAE和水提(AE)后样品的表面细胞结构. 结果表明,黄芪多糖的提取符合Fick第二定律,纤维素酶不改变提取传质动力学,仅破坏细胞壁,降低扩散阻力,使黄芪多糖的传质速率和有效扩散系数D¢明显提高. 实验得到D¢与温度T和APS粒径R0的关系,水提时为lnD¢=-9.30-926.7/T+1.76lnR0,酶提时为lnD¢=-10.56-491.4/T+1.75lnR0.

关键词: 黄芪多糖, 纤维素酶, 动力学, 提取

Abstract: The extraction of astragalus polysaccharides (APS) was studied with cellulase-assisted aqueous extraction (CAE) and aqueous extraction (AE). The effects of temperature (T) and particle size (R0) on the kinetic characteristics of extraction process were examined, and the cell microstructures of the samples extracted by CAE and AE were observed by SEM. The results showed that the extraction process of APS followed Fick model. Cellulase did not change the mass transfer mechanism of APS, but destroyed the structure of cells, and then significantly improved the mass transfer rate and apparent diffusion constant D¢. The relationships of D′ with temperature and particle size were expressed as lnD¢=-9.30-926.7/T+1.76lnR0 for AE, and lnD¢=-10.56-491.4/T+1.75lnR0 for CAE, respectively.

Key words: astragalus polysaccharide, cellulase, kinetics, extraction

中图分类号: