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›› 2005, Vol. 5 ›› Issue (6): 675-679.

• 生化工程专栏 • 上一篇    下一篇

一株青霉发酵产生木素过氧化物酶的工艺条件

阮芳勇,袁红莉   

  1. 中国农业大学生物学院
  • 出版日期:2005-12-20 发布日期:2005-12-20

Fermentation of Penicillium sp. to Produce Lignin Peroxidase

RUAN Fang-yong,YUAN Hong-li   

  1. College of Biological Science, China Agricultural University
  • Online:2005-12-20 Published:2005-12-20

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摘要： 研究了一株青霉菌P6 (Penicillium sp. P6)液体发酵产生木素过氧化物酶的工艺条件，考察了青霉菌P6在天然培养基稻米糠、玉米糠、麦糠和合成培养基GMY中的产酶情况，结果表明，麦糠是较理想的底物. 通过单因素方法研究了添加苯甲醇和MnSO4对酶活的影响；采用正交实验对MnSO4添加量、麦糠含量和培养基pH进行了研究，在实验范围内最佳酶活达到了2.69 U/mL. 进一步通过单双因素回归实验研究了麦糠和MnSO4的最佳含量，得到了最佳培养条件为孢子接种量1.1×106 mL-1，培养时间8 d，500 mL三角瓶装液量150 mL. 最佳培养基组成为麦糠50 g/L，初始pH 4.8, MnSO4 5.66 mmol/L.

关键词: 木素过氧化物酶(LiP), 青霉, 发酵工艺

Abstract: The optimal liquid-state fermentation conditions of Penicillium sp. P6 to produce lignin peroxidase were studied. The effects of rice bran, maize bran, wheat bran, GMY media, the volume of medium and temperature of culture were investigated. In addition, the effects of adding benzyl alcohol and MnSO4 to the media on LiP formation were also studied. The results of orthogonal experiments on the effects of supplemented MnSO4, wheat bran content and medium pH showed that the optimal conditions for LiP production were as follows: wheat bran 50 g/L, initial pH 4.8 and MnSO4 5 mmol/L, and the maximum activity of LiP reached 2.69 U/mL. The whole results showed that the suitable conditions were inoculum of 1.1×106 mL-1, fermentation time 8 d, added liquid volume ratio of 150 mL to 500 mL triangular flask, wheat bran content in medium of 50 g/L, pH 4.8 and MnSO4 5.66 mmol/L.

Key words: lignin peroxidase, Penicillium sp., fermentation