Cloning and Transcriptional Expression Analysis of ARE Gene in Rhodosporidium toruloides
ZHANG Guo-ling OURA Takahiro DU Wei LIU De-hua KAJIWARA Susum
Institute of Applied Chemistry，Department of Chemical Engineering, Tsinghua University Department of Life Science, Tokyo Institute of Technologytment of Chemical Engineering, Tsinghua University Department of Chemical Engineering, Tsinghua University Department of Chemical Engineering，Tsinghua University Department of Life Science，Tokyo Institute of Technology
Abstract：The ARE gene of Rhodosporidium toruloides was cloned and its transcriptional expression level analyzed. 2624 bp DNA sequence of ARE gene with 6 introns was clarified. The open reading frame was 2028 bp coding 675 amino acids. The transcriptional expression of ARE gene in the cells cultivated in nitrogen-limited medium increased with culture time. It was supposed that the transcriptional expression of ARE gene was related to lipid accumulation in R. toruloides.