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›› 2003, Vol. 3 ›› Issue (3): 0-0.

• 3 •    

两段双底物发酵生产1,3–丙二醇

杨东，李春，杜晨宇，张延平，曹竹安   

  1. 清华大学化学工程系生物化工研究所，北京 100084
  • 出版日期:2003-06-20 发布日期:2003-06-20

Production of 1,3-Propanediol by Klebsiella pneumoniae in Two Stage Two Substrate Fermentation

YANG Dong, LI Chun, DU Chen-yu, ZHANG Yan-ping, CAO Zhu-an   

  1. Dept. Chemical Engineering, Biochemical Engineering Institute, Tsinghua University, Beijing 100084, China
  • Online:2003-06-20 Published:2003-06-20

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摘要： 利用Klebsiella pneumoniae生长与催化耦合的特点，将好氧生长与厌氧转化两个过程耦合，开发了两段双底物发酵生产1,3-丙二醇的新工艺，并对其工艺特点进行了初步研究. 结果表明，采用两段双底物发酵工艺，发酵过程菌体浓度明显高于传统的厌氧转化工艺，OD650最大值达到9.37，发酵60 h, 1,3-丙二醇的浓度达到50.16 g/L，生成速率达0.836 g/(L×h)，比传统工艺提高了45%.

关键词: Klebsiella pneumoniae, 3-丙二醇, 甘油, 两段双底物发酵

Abstract: Based on the understanding that the cyanobacterial glycogen biosynthesis is a competitive pathway to cyanobacterial PHB biosynthesis, a genetically engineered cyanobacterium was constructed by partially replacing the agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis by an erythromycin resistance cassette. The obtained mutant propagated faster and the final biomass was higher against its wild-type species, which indicated a much higher photosynthetic efficiency in the mutant. No glycogen was detected in the mutant. Under the photoautotrophic condition, the intracellular PHB content was improved to 15% of the dry weight as compared to 3.4% in the wild-type strain.

Key words: Klebsiella pneumoniae, 3-propanediol, glycerol, two stage two substrate fermentation

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