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›› 2001, Vol. 1 ›› Issue (3): 0-0.

• 3 •    

褐藻糖胶的萃取和反萃

伍志春, 赵兵, 房燕丽, 欧阳藩   

  1. 1. 中国科学院化工冶金研究所, 北京 100080; 2. 中国科学院化工冶金研究所生化工程国家重点实验室,北京 100080
  • 出版日期:2001-06-20 发布日期:2001-06-20

Kinetic Analysis of Batch Fermentation of Streptomyces coelicolor

ZHANG He-chun, FAN Wei-min, ZHANG Yuan-xing   

  1. 1. Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China; 2. State Key Lab. Biochem. Eng., Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080, China
  • Online:2001-06-20 Published:2001-06-20

摘要: 研究了无机盐种类和浓度对N263萃取褐藻糖胶的影响. 结果表明,无机盐浓度是影响褐藻糖胶萃取的最主要因素, 褐藻糖胶的萃取率随盐浓度的增加而迅速降低,在无机盐存在下,增加萃取剂浓度并不能增加对褐藻糖胶的萃取. 用盐水溶液反萃不同条件下萃取的褐藻糖胶,结果表明,反萃率随盐浓度的增加而增加,在相同的氯离子浓度下,钠盐的反萃效率优于钙盐,随有机相中褐藻糖胶浓度的增加, 盐的反萃效率降低. 比较了不同溶剂作为稀释剂对用盐溶液反萃褐藻糖胶的影响,表明CCl4作为稀释剂时盐的反萃效率最低.

关键词: 褐藻糖胶, 萃取, 无机盐, 反萃取

Abstract: The effect of inorganic salt added into aqueous fucoidan solution on fucoidan extraction was investigated. It is found that extraction of fucoidan decreases rapidly with the increase of inorganic salt concentration in the aqueous solution. Based on this phenomenon, the stripping of fucoidan was carried out with aqueous salt solution. The experimental results show that the stripping of fucoidan increases with the concentration of inorganic salt in the aqueous solution and decreases with the increase of fucoidan concentration in the organic phase. In addition, the stripping efficiency of NaCl solution is higher than that of CaCl2 solution at the same chloride ion concentration. The effect of organic solvent as diluent on the stripping of fucoidan was also studied. It is shown that the stripping of fucoidan with carbon tetrachloride as diluent is more difficult than that with the other organic solvents.

Key words: kinetics, blue pigment, Streptomyces coelicolor, fermentation

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