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›› 2002, Vol. 2 ›› Issue (3): 0-0.

• 3 •    

高大毛霉制取果胶酶发酵条件实验

顾红燕,齐鸿雁,张洪勋   

  1. 中国科学院生态环境研究中心， 北京 100085
  • 出版日期:2002-06-20 发布日期:2002-06-20

Preparation of Zeolite NaA Membrane on Rough Porous a-Al2O3 Tube

LIANG Yu-xiu, LU Jin-ming, LI Bang-min, WANG Jin-qu, YIN De-hong   

  1. Research Center for Eco-envionmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
  • Online:2002-06-20 Published:2002-06-20

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摘要： 对高大毛霉(Mucor mucedo)制取果胶酶的发酵条件和酶的基本性质进行了研究. 发酵培养基组成为(g/L)：小麦麸皮50，葵盘粉30，(NH4)2SO4 30，KH2PO4 2.5，MgSO4×7H2O 0.5，NaNO3 0.2，FeSO4×7H2O 0.01. 在培养温度30℃、初始pH 5.7、转速240 r/min条件下摇瓶培养3 d，酶活力达到275 U/ml. 该酶最适pH为5.0，最适作用温度为40℃，在pH为3.0~7.0范围内稳定.

关键词: 高大毛霉, 果胶酶, 发酵条件

Abstract: The fermentation conditions of pectinase by Mucor mucedo and its enzymic properties were studied. The composition of fermentation medium was (g/L): wheat bran 50, sunflower calathide powders 30, (NH4)2SO4 30, KH2PO4 2.5, MgSO4×7H2O 0.5, NaNO3 0.2, FeSO4×7H2O 0.01. The pectinase activity can reach 275 U/ml after 3 d shaker culture under the conditions of 240 r/min, 30oC and initial pH 5.7. The optimum temperature and pH for the pectinase activity were 40oC and 5.0 respectively. The pectinase was stable in the pH range from 3.0 to 7.0.

Key words: zeolite NaA membrane, hydrothermal synthesis, separation property

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