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›› 2002, Vol. 2 ›› Issue (3): 0-0.

• 3 •    

红平红球菌LSSE8–1的培养及其对二苯并噻吩中硫元素脱除的影响

李珊,邢建民,缑仲轩,罗明芳,刘会洲,陈家镛   

  1. 中国科学院过程工程研究所分离科学与工程青年实验室及生化工程国家重点实验室，北京 100080
  • 出版日期:2002-06-20 发布日期:2002-06-20

Growth and Specific Desulfurization of a Newly Isolated Strain of Rhodococcus erythropolis

LI Shan, XING Jian-min, GOU Zhong-xuan, LUO Ming-fang, LIU Hui-zhou, CHEN Jia-yong   

  1. Youth Lab. Sep. Sci. Eng., State Key Lab. Biochem. Eng., Inst. Proc. Eng., CAS, Beijing 100080, China
  • Online:2002-06-20 Published:2002-06-20

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摘要： 红平红球菌(Rhodococcus erythropolis)LSSE8-1是一株新分离的专一性脱硫菌，HPLC分析表明该菌能选择性地脱除二苯并噻吩(DBT)中的硫，最终代谢产物是2-羟基联苯(2-HBP). 适宜的初始pH为6.5~9；能利用多种碳源，其中以甘油为最佳，适宜的甘油浓度为8 g/L；2 g/L的乙酸铵是菌体生长和脱硫的合适氮源；二甲基亚砜是提高该菌细胞收率和脱硫比活性的有效硫源.

关键词: 生物脱硫, 二苯并噻吩, 红平红球菌

Abstract: A newly isolated strain was identified as Rhodococcus erythropolis LSSE8-1, which can specifically remove sulfur from the model compound of dibenzothiophene (DBT) with 2-hydroxydiphenyl as the dead-end metabolic product. The optimal initial pH for growth varies from 6.5 to 7. Glycerol with the concentration of 8 g/L is the appropriate carbon source, and acetic ammonia at 2 g/L is suitable as nitrogen source for cell growth and desulfurization. It was found that R. erythropolis LSSE8-1 grew in the presence of sulfate or sulfur-containing amino acids but showed no desulfurization activity. Dimethylsulfoxide can enhance the cells yield and the activity of desulfurizing enzymes is not inhibited.

Key words: biodesulfurization, dibenzothiophene, Rhodococcus erythropolis

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