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›› 2014, Vol. 14 ›› Issue (2): 312-318.

• 生化工程专栏 • 上一篇    下一篇

里氏木霉Rut-C30产纤维素酶培养基优化及其酶解特性

张晓月 孜力汗 李勇昊 赵心清 白凤武   

  1. 大连理工大学生命科学与技术学院 大连理工大学生物科学与工程系 大连理工大学生物科学与工程系 大连理工大学生物科学与工程系 大连理工大学生物科学与工程系
  • 收稿日期:2013-12-20 修回日期:2014-03-10 出版日期:2014-04-20 发布日期:2014-04-20
  • 通讯作者: 孜力汗

Optimization of the Fermentation Medium for Cellulase Production from Trichoderma reesei Rut-C30 and Its Enzymatic Hydrolysis Characteristics

ZHANG Xiao-yue ZI Li-han， LI Yong-hao ZHAO Xin-qing, BAI Feng-wu   

  1. School of Life Science and Technology, Dalian University of Technology Department of Bioscience and Bioengineering , Dalian University of Technology Department of Bioscience and Bioengineering , Dalian University of Technology Department of Bioscience and Bioengineering, Dalian University of Technology Department of Bioscience and Bioengineering, Dalian University of Technology
  • Received:2013-12-20 Revised:2014-03-10 Online:2014-04-20 Published:2014-04-20
  • Contact: ZI Li-han，

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被引次数

16

摘要： 以廉价的工业纤维素诱导里氏木霉Rut-C30产纤维素酶，并对液体深层发酵培养基进行优化，采用响应面中心组合设计，以滤纸酶活为响应值，考察工业纤维素、麦麸、大豆粉浓度对纤维素酶活的影响. 结果表明，优化后的培养基组成为：工业纤维素35.62 g/L、麦麸19.37 g/L、大豆粉38.49 g/L，该条件下滤纸酶活达9.13 IU/mL，比优化前提高了72.26%，葡萄糖苷酶酶活提高了80.39%. 在121℃下用2% NaOH对玉米秸秆预处理45 min，物料中纤维素含量达64.94%，用该粗酶液酶解后酶解得率为94.68%.

关键词: 生物催化, 纤维素酶, 工业纤维素, 中心组合设计, 玉米秸秆

Abstract: Cheap industrial cellulose was taken as substrate for cellulase production from Trichoderma. reesei Rut-C30. The five-level three-variable central composite design implemented in the package of response surface methodology was adopted to evaluate the effects of industrial cellulose, wheat bran and soy flour on filter paper activity of (FPA) of cellulose. The results show that the optimal formulation of fermentation medium was determined as follows: industrial cellulose 35.62 g/L, wheat bran 19.37 g/L and soy flour 38.49 g/L. Thus, FPA achieved 9.13 IU/mL, which was increased by 72.26% over that without optimization, and the glucosidase activity was also increased by 80.39%. This crude cellulase solution was applied to obtain a hydrolysis yield of 94.68% in the hydrolysis of corn stalk which was pretreated by 2% NaOH for 45 min under 121℃, and the cellulose content of 64.94%.

Key words: biocatalysis, cellulase, industrial cellulose, central composite design, corn stalk

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