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›› 2014, Vol. 14 ›› Issue (2): 319-323.

• 生化工程专栏 • 上一篇    下一篇

微藻食用油脂浸提溶剂的选择与分析

王璐1 樊星 张冬梅 孙中亮 苏海佳 丛威   

  1. 北京化工大学北京市生物加工过程重点实验室 国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京工商大学食品学院 北京化工大学北京市生物加工过程重点实验室 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2014-02-24 修回日期:2014-04-08 出版日期:2014-04-20 发布日期:2014-04-20
  • 通讯作者: 王璐1

Selection and Exploration of Different Solvents for Extraction of Microalgae Edible Oil

WANG Lu FAN Xing ZHANG Dong-mei SUN Zhong-liang SU Hai-jia CONG Wei   

  1. Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology National Key Lab.Eng., Institute of Process Engineering, Chinese Academy of Sciences National Key Lab.Eng., Institute of Process Engineering, Chinese Academy of Sciences School of Food and Chemical Engineering, Beijing Technology and Business University Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., CAS
  • Received:2014-02-24 Revised:2014-04-08 Online:2014-04-20 Published:2014-04-20
  • Contact: WANG Lu

摘要: 分别向微藻中加入6种常用溶剂,冰浴超声处理15 min,振荡浸提8 h,提取微藻中的油脂,考察了溶剂的浸提能力. 结果表明,浸提干藻粉与湿藻泥中的油脂可选择不同溶剂,用正己烷浸提干藻粉中油脂,提取率可达71.52%,其中中性脂占75.86%,维生素含量E为1.63%,提取油脂后的藻渣中蛋白质、叶绿素和糖类的保留率分别为55.92%, 61.33%和78.35%,营养成分保留率较高;用正己烷/乙醇混合溶剂浸提湿藻泥中油脂,提取率可达68.31%,其中中性脂占71.65%,维生素E含量为1.87%,提取油脂后的藻渣中蛋白质、叶绿素和糖类的保留率分别为60.56%, 53.27%和80.20%.

关键词: 微藻, 油脂, 浸提, 溶剂, 中性脂, 维生素E, 藻渣

Abstract: Microalgae oil was extracted by six solvents commonly used for oil extraction, respectively, with ice bath ultrasonication for 15 min and extraction 8 h. The extraction behavior of microalgae oil with different solvents was studied. The results showed that for extraction of the oil from microalgae powder, n-hexane showed a better effect, resulting in a higher extraction rate of 71.52%, the oil contained 75.86% neutral lipid and 1.63% vitamin E, and the residual rates of protein, chlorophyll and carbohydrate were 55.92%, 61.33% and 78.35%, respectively. For extraction of the oil from microalgae ooze, n-hexane/ethanol mixed solvent showed a better effect, resulting in a higher extraction rate of 68.31%, the oil contained 71.65% neutral lipid and 1.87% vitamin E, and the residual rates of protein, chlorophyll and carbohydrate were 60.56%, 53.27% and 80.20%, respectively.

Key words: microalgae, oil, extraction, solvent, neutral lipid, vitamin E, residue

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