欢迎访问过程工程学报, 今天是

›› 2014, Vol. 14 ›› Issue (3): 469-475.

• 生化工程专栏 • 上一篇    下一篇

亲水改性聚氨酯纳米纤维酶膜催化油水两相体系转糖苷反应

栾盼盼 张羽飞 张松平 姜艳军 李鹏举 苏志国 高静   

  1. 河北工业大学化工学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 河北工业大学化工学院 河北工业大学化工学院 中国科学院过程工程研究所生化工程国家重点实验室 河北工业大学化工学院
  • 收稿日期:2014-03-06 修回日期:2014-04-11 出版日期:2014-06-20 发布日期:2014-06-20
  • 通讯作者: 栾盼盼

Transgalactosylation Reaction Catalyzed by Hydrophilic Modified PU Nanofibrous Enzyme Membrane in Oil-Water System

LUAN Pan-pan ZHANG Yu-fei, ZHANG Song-ping JIANG Yan-jun LI Peng-ju SU Zhi-guo GAO Jing   

  1. School of Chemical Engineering and Technology, Hebei University of Technology State Key Laboratory of Biochemical Engingeering, Institute of Process Engineering, Chinese Academy of Sciences National Key Laboratory of Biochemical Engingeering, Institute of Process Engineering, Chinese Academy of Sciences School of Chemical Engineering and Technology, Hebei University of Technology School of Chemical Engineering and Technology, Hebei University of Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences School of Chemical Engineering, Hebei University of Technology
  • Received:2014-03-06 Revised:2014-04-11 Online:2014-06-20 Published:2014-06-20
  • Contact: LUAN Pan-pan

摘要: 利用静电纺丝法制备含LiCl的聚氨酯(PU)纳米纤维,用牛血清白蛋白(BSA)对纤维亲水改性,于其上固定b-D-半乳糖苷酶,提高酶在油水两相介质中催化转糖苷反应的活力. 结果表明,以PU浓度26%(w)的电纺液制备的直径240~300 nm的PU纤维膜在LiCl辅助作用下吸附BSA高达222 mg/g,使纤维膜的水接触角由103.7o降至77.3o. 改性PU膜固定化酶比活力为1.59 U/mg,而未改性PU膜仅为其79.2%. 改性膜固定化酶55℃下的活力半衰期高达游离酶的14倍,在4℃下储存45 d活力仍保持80%,而游离酶活力仅剩余16.3%;改性膜固定化酶重复催化42次转糖苷反应活力仍能保持31%.

关键词: 静电纺丝, 聚氨酯纳米纤维, 半乳糖苷酶, 油水两相, 转糖苷反应, 亲水改性

Abstract: Polyurethane (PU) nanofiber with the diameter of 240~300 nm was prepared by electrospinning from 26%(w) PU solution containing LiCl. In order to increase the catalytic efficiency of b-D-galactosidase immobilized on PU nanofibrous membrane, suitable microenvironment on the membrane was pre-created by hydrophilic modification. BSA was adsorbed with LiCl on PU nanofibrous membrane at the high loading of 222 mg/g. The contact angle of modified PU nanofibrous membrane with water was thus reduced from 103.7o to 77.3o. Then b-D-galactosidase was immobilized on the modified membrane and catalyzed the transgalactosylation reaction in hexanol-water biphasic system. The specific activity of b-D-galactosidase immobilized on PU nanofiber membrane was 1.59 U/mg, while the activity of b-D-galactosidase adsorbed on unmodified PU nanofibrous membrane was its 79.2%. Compared with native enzyme, the half-life of b-D-galactosidase immobilized on PU nanofiber membrane incubated at 55℃ was increased 14 times. The storage stability of b-D-galactosidase immobilized on the membrane at 4℃ was also increased obviously, after storage for 45 d, the activity retained over 80%, whereas the activity of native b-D-galactosidase only 16.3%. The activity of b-D-galactosidase immobilized on PU nanofiber membrane was retained 31% after reuse in 42 times.

Key words: electrospinning, polyurethane nanofiber, β-D-galactosidase, oil-water system, transgalactosylation reaction, hydrophilic modification

中图分类号: