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›› 2007, Vol. 7 ›› Issue (4): 773-777.

• 生化工程专栏 • 上一篇    下一篇

大孔载体固定化脂肪酶

蔡宏举,付大雁,王满意,周鑫,谭天伟   

  1. 北京化工大学生命科学与技术学院
  • 出版日期:2007-08-20 发布日期:2007-08-20

Investigation of Immobilized Lipase onto Macroporous Carrier

CAI Hong-ju,FU da-yan,WANG Man-yi,ZHOU Xin,TAN Tian-wei   

  1. College of Life Science and Technology,Beijing University of Chemical Technology
  • Online:2007-08-20 Published:2007-08-20

摘要: 用自制大孔载体固定化脂肪酶,对固定化条件进行了优化,比较了固定化酶与游离酶的酶学参数. 结果表明,酶粉与载体质量比为1:1、固定化温度在20~25℃之间、固定化时间1.5 h的条件下,所得固定化酶的酶活最高. 固定化酶的最适pH为8.5,最适温度为40℃,其热稳定性、操作稳定性都比游离酶高,4℃下保存7 d后,酶活仍剩余94%.

关键词: 大孔载体, 脂肪酶, 固定化酶

Abstract: Immobilization of lipase on macroporous poly(glycidyl methacrylate-co-divinylbenzene) was investigated and followed by the optimization of immobilizing conditions. The optimized results were achieved with the 1:1 mass ratio of lipase to the carrier, coupling temperature between 20~25℃, and immobilizing time of 1.5 h. The optimum pH and temperature of immobilized lipase were 8.5 and 40℃, respectively. The activity of immobilized lipase remained 94% of the origin after storage at 4℃ for 7 d. The experimental results showed that the thermal stability and operational stability were improved in comparison with free lipase.

Key words: macroporous carrier, lipase, immobilized lipase