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›› 2009, Vol. 9 ›› Issue (2): 279-283.

• 反应与分离 • 上一篇    下一篇

Span-20和Tween-80对发酵液中Nisin泡沫分离的影响

李新涛 吴兆亮 赵艳丽 武亚明   

  1. 河北工业大学化工学院生物工程系 河北工业大学化工学院 河北工业大学化工学院 河北工业大学化工学院
  • 收稿日期:2008-10-14 修回日期:2008-12-03 出版日期:2009-04-20 发布日期:2009-04-20
  • 通讯作者: 吴兆亮

Effects of Span-20 and Tween-80 on Foam Fractionation Behavior of Nisin in Fermentation Broth

LI Xin-tao WU Zhao-liang, ZHAO Yan-li, WU Ya-ming   

  1. Department of Bioengineering, Hebei University of Technology Department of Bioengineering, Hebei University of Technology School of Chemical engineering, HeBei University of Technology School of Chemical engineering, HeBei University of Technology
  • Received:2008-10-14 Revised:2008-12-03 Online:2009-04-20 Published:2009-04-20
  • Contact: WU Zhao-liang,

摘要: 研究了微量非离子表面活性剂Tween-80和Span-20对发酵液中乳链菌肽(Nisin)泡沫分离的影响. 结果表明,发酵液泡沫分离时泡沫层轴向气泡直径、连续通气过程的泡沫层高度、泡沫层平均持气量等均因添加活性剂而明显变化. 不同体系气泡大小为,含0.2 g/L Span-20发酵液>含0.5 g/L Span-20发酵液>发酵液>含0.2 g/L Tween-80发酵液>含0.5 g/L Tween-80发酵液;Tween-80的加入破坏了气泡表面的网状结构,因而泡沫层持气量较高;连续通气时,2种表面活性剂都会引起泡沫层高度降低,但Tween-80会增加泡沫层的稳定性. 当Tween-80浓度达0.5 g/L时,Nisin的收率大幅度提高,原因是分离过程中Nisin失活率减少.

关键词: 泡沫分离, 非离子表面活性剂, 乳链菌肽

Abstract: The effects of Tween-80 and Span-20 on foam fractionation of Nisin (a food additive) fermentation broth were studied by using a single foam column. The results indicated that bubble size distribution, foam height and average gas holdup of the foam layer of the mixed system containing Tween-80 and Span-20 changed obviously from Nisin fermentation broth. The bubble size order of different mixed systems was: fermentation broth containing 0.2 g/L Span-20>fermentation broth containing 0.5 g/L Span-20>fermentation broth>fermentation broth containing 0.2 g/L Tween-80>fermentation broth containing 0.5 g/L Tween-80. The addition of surfactants decreased the height of foam layer and gas holdup, but Tween-80 increased the foam stability. And Tween-80 reduced the denaturation rate of Nisin in foam fractionation, so the separation effect was improved distinctly.

Key words: foam fractionation, nonionic surfactant, Nisin

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