疏水相互作用与亲水相互作用组合色谱纯化缩宫素

›› 2010, Vol. 10 ›› Issue (3): 598-602.

疏水相互作用与亲水相互作用组合色谱纯化缩宫素

张晓鸥宋茂谦王长海 Jan-Christer Janson 顾铭

大连理工大学环境与生命学院生物科学与工程系中国科学院过程工程研究所生化工程国家重点实验室大连理工大学环境与生命学院生物科学与工程系 Department of Physical and Analytical Chemistry, Uppsala Biomedical Centre, Uppsala University 中国科学院过程工程研究所生化工程国家重点实验室

收稿日期:2010-04-21 修回日期:2010-05-14 出版日期:2010-06-20 发布日期:2010-06-20
通讯作者: 张晓鸥

Purification of Oxytocin by Combining Hydrophobic Interaction Chromatography and Hydrophilic Interaction Chromatography

ZHANG Xiao-ou， SONG Mao-qian, WANG Chang-hai Jan-Christer Janson GU Ming

Department of bioscience and biotechnology, Dalian University of Technology Department of Physical and Analytical Chemistry, Uppsala Biomedical Centre, Uppsala University State Key Lab. Biochem. Eng., Inst. Process Eng., CAS

Received:2010-04-21 Revised:2010-05-14 Online:2010-06-20 Published:2010-06-20
Contact: ZHANG Xiao-ou，

摘要/Abstract

摘要： 将疏水相互作用色谱与亲水相互作用色谱结合，建立了用于化学合成缩宫素粗品分离纯化的两步纯化工艺. 先以纯水为流动相，采用Nylon 6疏水相互作用色谱对粗品进行脱盐与富集，后以Superose 12亲水相互作用色谱进行进一步纯化，两步纯化后，峰面积纯度达93.5%，提高了5.96倍，总回收率为50.1%，与用C18为介质的传统两步反相色谱纯化的结果相当. 本方法有机溶剂用量仅为传统反相色谱的1/7，且两种介质都能耐受极端pH值清洗条件，重复利用率高.

关键词: 聚己内酰胺, 琼脂糖凝胶, 疏水相互作用色谱, 亲水相互作用色谱, 缩宫素, 纯化

Abstract: A two-step process involving hydrophobic interaction chromatography and hydrophilic interaction chromatography was developed to purify chemically synthetic oxytocin out of economic and environmental consideration. First, Nylon 6 hydrophobic interaction chromatography was performed using water as mobile phase for desalting and enrichment. And Superose 12 hydrophilic interaction chromatography was performed for further purification. By this two-step purification process, the purity of oxytocin was increased up to 93.5% (5.96 folds purification) with final recovery rate of 50.1%, which was comparable with the results obtained from traditional two-step RPLC purification process with C18 medium. This two-step purification process consumed less organic solvent, which meant lower cost. Nylon 6 and Superose 12 media were chemically stable under extreme pH cleaning conditions and easily reproduced.

Key words: polycaprolactam, agarose gel, hydrophobic interaction chromatography, hydrophilic interaction liquid chromatography, oxytocin, purification

中图分类号:

张晓鸥宋茂谦王长海 Jan-Christer Janson 顾铭. 疏水相互作用与亲水相互作用组合色谱纯化缩宫素[J]. , 2010, 10(3): 598-602.

ZHANG Xiao-ou; SONG Mao-qian; WANG Chang-hai Jan-Christer Janson GU Ming. Purification of Oxytocin by Combining Hydrophobic Interaction Chromatography and Hydrophilic Interaction Chromatography[J]. , 2010, 10(3): 598-602.

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