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›› 2002, Vol. 2 ›› Issue (6): 0-0.

• 6 •    

脂肪酶不对称立体选择性能改善的研究进展

聂尧，徐岩，王栋   

  1. 江南大学生物工程学院教育部工业生物技术重点实验室，江苏 无锡 214036
  • 出版日期:2002-12-20 发布日期:2002-12-20

Progress in Improving the Asymmetric Enantioselectivity of Lipase for Catalyzing Asymmetric Reaction

NIE Yao, XU Yan，WANG Dong   

  1. Key Lab. Ind. Biotech., Ministry of Edu., School of Biotech., Southern Yangtze Univ., Wuxi, Jiangsu 214036, China
  • Online:2002-12-20 Published:2002-12-20

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被引次数

25

摘要： 脂肪酶已广泛用于制备光学纯手性化合物的不对称反应中，但大多数酶催化拆分外消旋化合物的立体选择性不很理想. 目前，改善脂肪酶立体选择性的研究主要从改造脂肪酶酶蛋白结构、优化体系的反应条件、改善反应过程以及对映选择性抑制等方面进行. 通过微波照射也能在一定程度上改善脂肪酶催化反应的立体选择性. 本文主要介绍了几种改善脂肪酶催化不对称反应的立体选择性的方法.

关键词: 脂肪酶, 立体选择性, 不对称反应

Abstract: Lipase has been extensively used in asymmetric reaction for producing optically pure compounds. Focused on improving the enantioselectivity of lipase for catalyzing asymmetric reaction, the recent researches have been summarized in this article, including reformation of lipase structure, reaction condition optimization, improvement of asymmetric reaction process and enantioselective inhibitation. Microwave irradiation can also be used to enhance the enantioselectivity of lipase in some level. Some traditional and new approaches, such as immobilization of enzyme, chemical modification, in vitro directed evolution, bio-imprinting, and optimization of reaction conditions were introduced.

Key words: lipase, enantioselectivity, asymmetric reaction

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